In vitro development of canine somatic cell nuclear transfer embryos in different culture media |
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| Authors: | Dong-Hoon Kim Jin-Gu No Mi-Kyung Choi Dong-Hyeon Yeom Dong-Kyo Kim Byoung-Chul Yang Jae Gyu Yoo Min Kyu Kim Hong-Tea Kim |
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| Institution: | 1Division of Animal Biotechnology, National Institute of Animal Science, Rural Development Administration, Suwon 441-706, Korea.;2Division of Dairy Science, National Institute of Animal Science, Rural Development Administration, Suwon 441-706, Korea.;3Department of Animal Science and Biotechnology, Chungnam National University, Daejeon 305-764, Korea.;4Department of Biological Science, Sungkyunkwan University, Suwon 440-746, Korea. |
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| Abstract: | The objective of the present study was to investigate the effects of three different culture media on the development of canine somatic cell nuclear transfer (SCNT) embryos. Canine cloned embryos were cultured in modified synthetic oviductal fluid (mSOF), porcine zygote medium-3 (PZM-3), or G1/G2 sequential media. Our results showed that the G1/G2 media yielded significantly higher morula and blastocyst development in canine SCNT embryos (26.1% and 7.8%, respectively) compared to PZM-3 (8.5% and 0%) or mSOF (2.3% and 0%) media. In conclusion, this study suggests that blastocysts can be produced more efficiently using G1/G2 media to culture canine SCNT embryos. |
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| Keywords: | canine G1/G2 media in vitro culture somatic cell nuclear transfer embryo |
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