| 甘蔗黄叶病毒的TBIA快速检测 |
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| 引用本文： | 李文凤,;黄应昆,;王晓燕,;陈学宽,;卢文洁,;吴才文,;罗志明.甘蔗黄叶病毒的TBIA快速检测[J].中国甜菜,2009(3):22-23. |
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| 作者姓名： | 李文凤 ;黄应昆 ;王晓燕 ;陈学宽 ;卢文洁 ;吴才文 ;罗志明 |
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| 作者单位： | [1]云南省农业科学院甘蔗研究所,开远661600; [2]云南省甘蔗遗传改良重点实验室,开远661600 |
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| 基金项目： | 云南省科技攻关及高新技术发展计划项目（2006NG20）;公益性行业（农业）科研专项（nyhyzx07-019）;农业部“948”项目（2006-G37）;现代农业产业技术体系建设专项. |
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| 摘 要： | 利用从法国引进的甘蔗黄叶病毒（SCYLV）标准抗原抗体，研究建立了TBIA快速检测SCYLV的方法。通过对田间采集的样本进行检测，同时以DAS—ELISA检测法印证，检测结果一致，表明TBIA能简便、快速、准确、有效地检测出SCYLV，且检测效率高，适合于田间大批量样品快速检测。为甘蔗黄叶病的诊断和防治、脱毒种苗的生产、对外甘蔗品种／材料交换检疫检测提供了技术支撑。
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| 关 键 词： | 甘蔗黄叶病 TBIA 检测 |
Rapid Detection of Sugarcane Yellow Leaf Syndrome Disease by TBIA |
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| Institution: | LI Wen-feng, HUANG Ying-kun, WANG Xiao-yan, CHEN Xue-kuan, LU Wen-jie, WU Cai-wen, LUO Zhi-ming (Sugarcane Research Institute, Yunnan Province Academy of A gricultural Sciences/Yunnan Key Laboratory of Sugarcane Genetic Imp rove me nt ,K aiyuan 661600,China) |
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| Abstract: | The detecting method of TBIA for sugarcane yellow leaf syndrome （ScYLV） is based on standard antigen and antibody from French. The ScYLV can be rapidly, conveniently and truly detected by the TBIA for field samples and the testing results are as the same as the method of negative staining of DAS-ELISA. The TBIA method of provides a technique support seedling and quarantine of ScYLV for exc for diagnosis and control of ScYLV, production of sugarcane virus-free hanged sugarcane materials. |
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| Keywords: | Sugarcane yellow leaf syndrome disease Tissue blot immunoassay Detection |
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