| 野生西藏虎头兰组培技术研究 |
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| 引用本文： | 蓝玉甜,吴天贵,刘世勇,黄岚,罗玉婷,韦新莲.野生西藏虎头兰组培技术研究[J].福建林业科技,2010,37(1). |
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| 作者姓名： | 蓝玉甜 吴天贵 刘世勇 黄岚 罗玉婷 韦新莲 |
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| 作者单位： | 广西雅长兰科植物自然保护区管理局,广西,乐业,533209 |
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| 基金项目： | 科技部公益项目“广西雅长兰科种子基因库营建与可持性利用”(项目编号:2005DJB6J114) |
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| 摘 要： | 以野生西藏虎头兰种子为试验材料,应用组培技术开展种子无菌播种、原球茎诱导芽分化、试管苗生根等试验,初步筛选出不同发育阶段的培养基配方。种子萌发培养基为:Hyponex 1号1 g.L-1+Hyponex 2号1 g.L-1+6-BA1 mg.L-1+10%香蕉汁+2%苹果汁+蔗糖20 g.L-1+琼脂6 g.L-1+AC 1 g.L-1,种子萌发率达90%～98%;原球茎分化最佳培养基为:MS+NAA1 mg.L-1+5%香蕉汁+2%苹果汁+蔗糖20 g.L-1+琼脂6 g.L-1+AC1 g.L-1,芽分化率高达89%;壮苗生根最佳培养基为:1/2 MS+NAA1 mg.L-1+5%香蕉汁+AC 1.5 g.L-1+蔗糖20 g.L-1+琼脂6 g.L-1,生根率达100%。
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| 关 键 词： | 西藏虎头兰 种子萌发 原球茎分化 生根培养 |
Study on Tissue Culture of Wild Cymbidium tracyanum L.Castler. |
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| LAN Yu-tian,WU Tian-gui,LIU Shi-Yong,HUANG Lan,LUO Yu-ting,WEI Xin-lian.Study on Tissue Culture of Wild Cymbidium tracyanum L.Castler.[J].Journal of Fujian Forestry Science and Technology,2010,37(1). |
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| Authors: | LAN Yu-tian WU Tian-gui LIU Shi-Yong HUANG Lan LUO Yu-ting WEI Xin-lian |
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| Institution: | Guangxi Yachang Orchidaceae Nature Reserve Administrative Bureau;Leye 533209;Guangxi;China |
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| Abstract: | Taking wild Cymbidium tracyanum L.Castle seeds as materials,the tissue culture technology was applied to carry out sterile seed sowing,the original bulb induced bud differentiation,plantlet rooting etc.And the medium formula at different developmental stages was preliminary screened.The seed germination medium was:1g of No.1 Hyponex/L+1 g of No.2 Hyponex/L+1 mg of 6-BA/L+10% of banana juice+2% of apple juice+20 g of sucrose/L+6 g of agar/L+1 g of AC/L,seed germination rate was 90%—98%;the best culture medium for the original bulb differentiation was: MS+1 mg of NAA/L+5% of banana juice+2% of apple juice+20 g of sucrose/L+6 g of agar/L +1 g of AC/L,bud differentiation rate was 89%;the best culture medium for the strong seedling rooting was:1/2MS+ 1 mg of NAA/L+5% of banana juice +1.5g of AC/L+20g of sucrose/L+6 g of agar/L,rooting rate was 100%. |
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| Keywords: | Cymbidium tracyanum L Castle seed germination the original bulb differentiation rooting culture |
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