| 间接免疫荧光染色空斑计数技术的建立及应用 |
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| 引用本文： | 吴长新,刘秀梵,张如宽,甘军纪.间接免疫荧光染色空斑计数技术的建立及应用[J].扬州大学学报(农业与生命科学版),1994(4). |
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| 作者姓名： | 吴长新 刘秀梵 张如宽 甘军纪 |
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| 作者单位： | 江苏农学院畜牧兽医系 |
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| 摘 要： | 以间接免疫荧光抗体技术（ＩＦＡ）为基础，并加以改进，建立了间接免疫荧光染色空斑计数技术（ＰＣＴＩＩＦＳ），用其检测鸡马立克氏病（ＭＤ）二价疫苗，可快速准确地计数疫苗中不同血清型病毒各自的空斑形成单位（ＰＦＵ）及其ＰＦＵ总数。克服了常规法不能计数二价苗中两种病毒各自空斑数的缺点，敏感性显著高于常规病毒空斑计数法。
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| 关 键 词： | 间接法＋免疫荧光法 计数 特异性 灵敏度／空斑 |
ESTABLISHMENT OF PLAQUE-COUNTING TECHNIC BYINDIRECT IMMUNOFLUORESENT STAINING AND IT'SPRELIMINARY APLICATION |
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| Wu Changxin,Liu Xiufan,Zhang Rukuan,Gan Junji.ESTABLISHMENT OF PLAQUE-COUNTING TECHNIC BYINDIRECT IMMUNOFLUORESENT STAINING AND IT''''SPRELIMINARY APLICATION[J].Journal of Yangzhou University:Agricultural and Life Science Edition,1994(4). |
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| Authors: | Wu Changxin Liu Xiufan Zhang Rukuan Gan Junji |
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| Abstract: | A new method counting virus plaque form unit(PFU)called Plaque-Counting Technic by In-direct Immunofluoresent Staining(PCTIIFS)was established.Compared with traditional virus PFU countingmethod,PCTIIFS characterized higher sensitivity,and specificity,better reproduciblity and more rapidity.Significantly,PCTIIFS could count PFU of two or more serotypes or viruses strains in one specimen simulta-neously and respectively,such as bivalent or trivalent vaccine containing two or three serotype viruses.Pre-liminary aplication showed PCTIIFS could accurately count viruses PFU of MD bivalent vaccine containing Z_4and F_(c126) respectively. |
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| Keywords: | indireet methods+immuno fluoresence method stains and staining(biology) counting specificity secentivity/plaque |
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