| 苹果绵蚜线粒体DNA的提取方法研究简报 |
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| 引用本文： | 李美,杨勤民,甚爱东,赵兴波,张龙.苹果绵蚜线粒体DNA的提取方法研究简报[J].云南农业大学学报,2007,22(1):141-142. |
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| 作者姓名： | 李美 杨勤民 甚爱东 赵兴波 张龙 |
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| 作者单位： | [1]中国农业大学,农业部害虫生物防治重点实验室,北京100094 [2]山东省植物保护总站,山东济南250000 [3]云南省农业科学院植物保护所,云南昆明650205 [4]中国农业大学,农业生物技术国家重点实验室,北京100094 |
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| 摘 要： | 获得高质量的线粒体DNA（mtDNA）是进行mtDNA研究的前提。本研究用碱裂解法和加热法均得到了满足RFLP分析要求的苹果绵蚜mtDNA。但只有碱裂解法提取的mtDNA满足克隆的需要。经分析认为,在实验过程中尽可能的避免核DNA的断裂,是获取高质量mtDNA的关键。
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| 关 键 词： | 苹果绵蚜 线粒体DNA 碱裂解法 |
| 文章编号： | 1004-390X（2007）01-0141-02 |
| 收稿时间： | 2006-4-27 |
| 修稿时间： | 2006-04-27 |
Methods of Isolating MtDNA from Woolly Apple Aphids Eriosoma langerum (Hausmann) |
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| LI Mei,YANG Qin-min,SHEN Ai-dong,ZHAO Xing-bo,ZHANG Long.Methods of Isolating MtDNA from Woolly Apple Aphids Eriosoma langerum (Hausmann)[J].Journal of Yunnan Agricultural University,2007,22(1):141-142. |
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| Authors: | LI Mei YANG Qin-min SHEN Ai-dong ZHAO Xing-bo ZHANG Long |
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| Institution: | 1. The Key Laboratory for Biological Control of Ministry of Agriculture, China Agricultural University, Beijing 100094, China; 2. Shandong Gengeral Station of Plant Protection, Jinan 250100, China; 3. Institute of Plant Protection, Yunnan Academy of Agricultural Sciences, Kunming 650205, China; 4. National State Key Lab for Agricuhure Biotechnology Sciences, China Agricultural University, Beijing 100094, China |
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| Abstract: | Top-quality mitochondrial DNA are required in studies of mitochondrial genome.MtDNA of woolly apple aphids Eriosoma langerum(Hausmann),which can be used in Restriction fragment length polymorphism (RFLP) analysis,are isolated with both methods of alkaline lysis and calefaction.But if the sequences of mtDNA of woolly apple aphids are to be cloned,only the method of alkaline lysis is suit.In the progress of isolating the mtDNA,avoiding the break of nuclear DNA is the key. |
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| Keywords: | Woolly apple aphid(Eriosoma langerum) mitochondrial DNA methods of alkalinelysis |
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