| 绵羊附睾尾部精液低温保存效果的研究 |
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| 引用本文： | 高建明,王占赫,王保利,张中文,张银花,胡麦顺.绵羊附睾尾部精液低温保存效果的研究[J].北京农学院学报,2004,19(2):39-42. |
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| 作者姓名： | 高建明 王占赫 王保利 张中文 张银花 胡麦顺 |
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| 作者单位： | 1. 北京农学院动物科学技术系,北京,102206
2. 北京怀柔区畜牧水产服务中心,北京,101400 |
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| 摘 要： | 由成年绵羊离体睾丸上获得附睾尾部精液,进行3种浓度稀释液(葡萄糖、柠檬酸三钠、卵黄含量分别为:Ⅰ组1 5g,0 7g,10ml;Ⅱ组0 4g,1 4g,10ml;Ⅲ组1 5g,0 7g,4ml)低温保存(4℃)效果的观察,并对低温保存前精液的处理方法进行了比较。结果表明:Ⅲ组绵羊精液低温保存总存活时间和存活指数均好于Ⅰ、Ⅱ两组(P<0 01;P<0 05);Ⅲ组保存前经上游处理的精液在总存活时间、存活指数和体外受精效果均明显高于未经上游处理的精液。
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| 关 键 词： | 绵羊 附睾尾精液 低温保存 稀释液 精子上游 |
| 文章编号： | 1002-3186(2004)02-0039-04 |
| 修稿时间： | 2003年12月8日 |
Effect of Low Temperature Preservation on the Sheep Semen from Epididymis Tail |
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| GAO Jian-ming,WANG Zhan-he,WANG Bao-li,ZHANG Zhong-wen,ZHANG Yin-hua,HU Mai-shun.Effect of Low Temperature Preservation on the Sheep Semen from Epididymis Tail[J].Journal of Beijing Agricultural College,2004,19(2):39-42. |
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| Authors: | GAO Jian-ming WANG Zhan-he WANG Bao-li ZHANG Zhong-wen ZHANG Yin-hua HU Mai-shun |
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| Institution: | GAO Jian-ming~1,WANG Zhan-he~1,WANG Bao-li~2,ZHANG Zhong-wen~1,ZHANG Yin-hua,HU Mai-shun~1 |
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| Abstract: | The epididymis tail semen from adult sheep testicle were observed after low temperature preservation (4 ℃) with three different semen diluents (the components of glucose, tri-sodium citric acid and egg yolk were 1.5g, 0.7g, 10ml; 0.4g, 1.4g, 10ml and 1.5g, 0.7g, 4ml for groups Ⅰ, Ⅱ and Ⅲ, respectively) and two handling methods of semen (swim-up and non-swim-up) were compared before low temperature preservation. The results showed that the total survival time and survival index of semen in group Ⅲ were significantly better than group Ⅰand Ⅱ (P<0.01, P<0.05). The total survival time and survival index and the effect of group Ⅲ semen on in vitro fertilization with swim-up method were better than those with non-swim-up method. |
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| Keywords: | sheep epididymis tail low temperature preservation diluents sperm swim-up |
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