| 牛早期胚胎PCR法性别鉴定条件的优化 |
| |
| 引用本文： | 赵 雪,王保莉,安志兴.牛早期胚胎PCR法性别鉴定条件的优化[J].西北农林科技大学学报(社会科学版),2006,34(9):27-30. |
| |
| 作者姓名： | 赵 雪 王保莉 安志兴 |
| |
| 作者单位： | 西北农林科技大学,生物工程研究所,陕西,杨凌,712100 |
| |
| 基金项目： | 国家高技术研究发展计划(863计划) |
| |
| 摘 要： | 对牛早期胚胎性别鉴定PCR反应体系及反应程序进行了优化。结果显示,选用双套引物和连续复合PCR方法,先用雄性特异性引物进行10个循环,然后再加入牛特异性引物进行22个循环,共32个循环,可实现两种基因片段的同时扩增,保证了PCR的准确性。胚胎移植结果显示该方法准确可靠。
|
| 关 键 词： | 胚胎 PCR 性别鉴定 牛 |
| 文章编号： | 1671-9387(2006)09-0027-04 |
| 收稿时间： | 2006-03-20 |
| 修稿时间： | 2006年3月20日 |
The PCR optimization the sex identification of bovine embryos |
| |
| ZHAO Xue,WANG Bao-li,AN Zhi-xing,ZHANG Yong.The PCR optimization the sex identification of bovine embryos[J].Journal of Northwest Sci-Tech Univ of Agr and,2006,34(9):27-30. |
| |
| Authors: | ZHAO Xue WANG Bao-li AN Zhi-xing ZHANG Yong |
| |
| Institution: | (Institute of Bio-Engineering,Northwest A & F University,Yangling,Shaanxi 712100,China) |
| |
| Abstract: | The objective of this study was to establish the optimum PCR condition for the sexing of bovine early embryos,the optimum PCR procotol,in consecutive and multiplex PCR,the first 10 PCR cycles were done with male-specific primer followed by an additional 22-cycles with bovine-specific primer. The sexes of 21 bovine early embryos were identified and the identified efficiency of bovine early embryos was 100% according to the result of embryo transfer. |
| |
| Keywords: | PCR |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《西北农林科技大学学报(社会科学版)》浏览原始摘要信息 |
| 点击此处可从《西北农林科技大学学报(社会科学版)》下载免费的PDF全文 |
|
