| 用SSR标记提高水稻分子连锁图谱密度 |
| |
| 引用本文： | 段远霖,赵守环,吴为人,周元昌,祁建民,潘润森,林荔辉,陈志伟,官华忠,毛大梅.用SSR标记提高水稻分子连锁图谱密度[J].分子植物育种,2003,1(4):475-479. |
| |
| 作者姓名： | 段远霖 赵守环 吴为人 周元昌 祁建民 潘润森 林荔辉 陈志伟 官华忠 毛大梅 |
| |
| 作者单位： | 1. 福建农林大学作物学院,福州,350002
2. 福建农林大学作物学院,福州,350002;浙江大学农业与生物技术学院,杭州,310029 |
| |
| 摘 要： | 本课题组利用圭630／台湾粳的DH群体，建立了一个水稻RFLP连锁图。该图谱含175个标记，总长1224．6cM，相邻标记间平均距离为7．0cM。但该图标记分布不够均匀，存在较多空白区，且在第4和第8染色体上分别存在一个断点。本研究试图在原有RFLP图谱的基础上，添加一些SSR标记，以使该图谱标记更加密集和均匀，并消除两个断点。共筛选了361对RM引物，获得了183对多态标记，在12条染色体上共整合了57个RM标记，染色体连锁图总长度为1811．2cM，比原图谱增长了47．9％，相邻标记间平均距离为7．8cM，与原图谱相近。不过，两条染色体上的两个断点仍无法消除，暗示这两个断点区域多态性较低或重组率较高。
|
| 关 键 词： | 分子标记辅助育种 水稻 分子连锁图谱 RFLP SSR 遗传图谱 |
Saturating a Molecular Linkage Map of Rice with SSR Markers |
| |
| Duan Yuanlin Zhao Shouhuan Wu Weiren , Zhou Yuanchang Qi Jianmin Pan Runsheng Lin Lihui Chen Zhiwei Guan Huazhong Mao Damei College of Crop Science,Fujian Agriculture and Forestry University,Fuzhou, College of Agriculture and Biotechnology,Zhejiang University,Hangzhou, These authors contributed equally to this work Corresponding author,zjweiren@pub fz fj cn.Saturating a Molecular Linkage Map of Rice with SSR Markers[J].Molecular Plant Breeding,2003,1(4):475-479. |
| |
| Authors: | Duan Yuanlin Zhao Shouhuan Wu Weiren Zhou Yuanchang Qi Jianmin Pan Runsheng Lin Lihui Chen Zhiwei Guan Huazhong Mao Damei College of Crop Science Fujian Agriculture and Forestry University Fuzhou College of Agriculture and Biotechnology Zhejiang University Hangzhou These authors contributed equally to this work Corresponding author zjweiren@pub fz fj cn |
| |
| Institution: | Duan Yuanlin 1* Zhao Shouhuan 1* Wu Weiren 1,2** Zhou Yuanchang 1 Qi Jianmin 1 Pan Runsheng 1 Lin Lihui 1 Chen Zhiwei 1 Guan Huazhong 1 Mao Damei 1 1 College of Crop Science,Fujian Agriculture and Forestry University,Fuzhou,350002 2 College of Agriculture and Biotechnology,Zhejiang University,Hangzhou,310029 * These authors contributed equally to this work ** Corresponding author,zjweiren@pub5 fz fj cn |
| |
| Abstract: | We had constructed an RFLP marker lingkage map of rice based on a doubled haploid (DH) population derived from a cross between an indica rice variety Gui-630 and a japonica rice variety Taiwanjing. The map contains 175 RFLP markers and covers a total length of 1224 6cM with an average distance of 7 0cM between adjacent markers. But markers on the map are not evenly distributed and there are two breaking points on chromosomes 4 and 8. This study aimed to avoid the two breaking points and make markers distributed denser and more evenly in the map by adding some SSR markers. A total of 361 pairs of SSR primers were used to screen polymorphic markers between the parents. 183 pairs of the primers showed polymorphism and 57 SSR markers were added into the map. The new map covers a total length of 1811 2cM, 47 9% longer than the original map; the average distance between adjacent markers is 7 8cM, similar to the original map. However, the two broken points still exist, implying that the two regions might be less polymorphic and of high recombination frequency. |
| |
| Keywords: | Rice ( O sativa L ) SSR RFLP Genetic map |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
