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奶牛日粮添加豆油抑制乳腺硬脂酰辅酶A去饱和酶(SCD)基因mRNA表达水平
引用本文:卜登攀,王加启,刘仕军,刘晓云,谷俊,张瑞超.奶牛日粮添加豆油抑制乳腺硬脂酰辅酶A去饱和酶(SCD)基因mRNA表达水平[J].中国畜牧兽医,2006,33(7):3-5.
作者姓名:卜登攀  王加启  刘仕军  刘晓云  谷俊  张瑞超
作者单位:中国农业科学院北京畜牧兽医研究所动物营养学国家重点实验室,北京,100094
基金项目:科技部国家“十五”科技攻关计划“奶业重大关键技术研究与产业化技术集成示范”(2002BA51802)
摘    要:硬脂酰辅酶A去饱和酶(SCD)是奶牛乳腺合成共轭亚油酸(CLA)的关键酶。奶牛日粮饲喂35 d豆油后,与不添加豆油的对照组相比,抑制了奶牛乳腺组织SCD基因mRNA的表达,研究结果表明,日粮亚油酸对乳腺SCD酶基因表达具有抑制作用。

关 键 词:豆油  硬脂酰辅酶A去饱和酶(SCD)  基因表达  实时定量PCR(RT-PCR)
文章编号:1671-7236(2006)07-0003-02
收稿时间:2006-06-09

Addition of Soybean Oil to Diets for Dairy Cows Decrease SCD Gene Expression in Mammary Gland
BU deng-pan,WANG jia-qi,LIU shi-jun,LIU xiao-yun,GU jun,ZHANG rui-chao.Addition of Soybean Oil to Diets for Dairy Cows Decrease SCD Gene Expression in Mammary Gland[J].China Animal Husbandry & Veterinary Medicine,2006,33(7):3-5.
Authors:BU deng-pan  WANG jia-qi  LIU shi-jun  LIU xiao-yun  GU jun  ZHANG rui-chao
Institution:Institute of Aniaml Science,State Key laboratory of animal Nutrition,Beijing 100094
Abstract:The experiment was conducted to determine the effect of dietary soybean oil on Stearoyl-CoA-desaturase(SCD) gene expression in mammary gland.Multiparous Hostein cows(n=6)in middle lactation were used in a completely randomized design.Cows were given a total mixed ration with no added soybean oil(CTL) or soybean oil(TRT,about 2% of dry matter) for 5 weeks.Mammary gland tissue was taken from a rear quarter at the end of the treatment period.Consumption of soybean oil decreased the abundance of SCD mRNA in the mammary gland by 50% compared to control group.The results suggest that soybean oil decrease expression of SCD gene mainly due to the intake of linoleic acid.
Keywords:soybean oil  stearoyl-coa-desaturase(SCD)  gene expression  RT-PCR  
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