| 拟南芥热激因子HSF1的表达与纯化 |
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| 引用本文： | 郭丽红,王定康,袁燕,刘开庆,张乐民.拟南芥热激因子HSF1的表达与纯化[J].安徽农业科学,2009,37(24):11444-11445. |
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| 作者姓名： | 郭丽红 王定康 袁燕 刘开庆 张乐民 |
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| 作者单位： | 昆明学院生命科学与技术系,云南昆明,650031;云南大学生物资源保护与利用重点实验室,云南昆明,650092 |
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| 基金项目： | 国家自然科学基金项目，云南省面上基金项目，云南省教育厅项目 |
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| 摘 要： | 目的]表达和纯化拟南芥热激因子HSF1。方法]以构建的能表达热激因子HSF1的大肠杆菌Escherichia coli M15（pQE32／His6-HSF1，pREP4）为材料，用异丙基硫代-β—D-半乳糖苷（IPTG）诱导表达HSF1，再通过镍亲和层析纯化表达的HSF1，通过变性的聚丙酰胺（SDS．PAGE）电泳分析表达蛋白和纯化蛋白。结果]试验获得了表达的HSF1，并且进一步获得了纯化的HSF1。结论]该研究为探讨拟南芥HSF1在基因组的结合位点提供了试验材料，为全面认识HSF1作用机理和生理功能奠定了基础。
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| 关 键 词： | 拟南芥热激因子HSF1 表达 纯化 |
Expression and Purification of Heat Shock Factor HSF1 in Arabidopsis thaliana |
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| GUO Li-hong et al.Expression and Purification of Heat Shock Factor HSF1 in Arabidopsis thaliana[J].Journal of Anhui Agricultural Sciences,2009,37(24):11444-11445. |
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| Authors: | GUO Li-hong |
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| Institution: | GUO Li-hong et al(Department of Life Science , Technology,Kunming University,Kunming,Yunan 650031) |
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| Abstract: | Objective] This study was to express and purify Arabidopsis thaliana heat shock factor HSF1.Method] Using Escherichia coli M15 harboring HSF1(pQE32/His6-HSF1,pREP4) as experimental materials,HSF1 was induced to express with isopropyl-β-D-galactoside(IPTG);then the expression product was purified using Ni-NTA-agarose affinity chromatography and analyzed by SDS-PAGE.Result] HSF1 of Arabidopsis thaliana was successfully expressed and purified.Conclusion] This study provided materials for understanding the binding site of HSF1 on Arabidopsis thaliana chromosome,further laying a good foundation for revealing the regulatory mechanism and physiological function of HSF1. |
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| Keywords: | Heat Shock Factor HSF1 Expression Purification |
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