Identification of sweet cherry cultivars (Prunus avium L.) and analysis of their genetic relationships by chloroplast sequence-characterised amplified regions (cpSCAR) |
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| Authors: | Aydin Turkec Muge Sayar Berthold Heinze |
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| Institution: | (1) Mustafa Kemalpasa Vocational School, Uludag University, 16500 Bursa, Turkey;(2) Department of Molecular Biology and Genetics, Bogazici University, 34342 Bebek, Istanbul, Turkey;(3) Federal Research Centre for Forestry, Hauptstrasse 7, A-1140 Vienna, Austria |
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| Abstract: | Ten cpSCAR markers that show polymorphism in Prunus avium were used to fingerprint sweet cherry cultivars. The purpose of the study was also to contribute to identification and to
help determine their genetic interrelationships. Samples of ‘0900 Ziraat’, a superior Turkish variety, which were collected
in several locations all over Turkey, had identical cpSCAR patterns, and they resembled a common European haplotype, A. ‘Sweetheart’,
‘Summit’ and ‘Canada Giant’ and their haplotype are intermediate between the previously described haplotypes A and B, which
were originally found in Central and Eastern European sweet and wild cherries, and those from Northern Turkey, respectively.
The data therefore suggests a local maternal descent (within Europe and Asia Minor) of the cultivars analysed. Our results
show that chloroplast DNA analysis is a straightforward way to classify cherry cultivars. We compare our results to others
previously reported for sweet cherry cultivars, and conclude that cpSCAR diversity data could be considered for phylogenetic
studies in this group. |
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| Keywords: | cpDNA primers Genetic diversity Haplotype PCR– RFLP Polymorphism Prunus avium |
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