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Genetic segregation of the deformed flower gene in trisomics of Solanum chacoense
Authors:Heiyoung K Lee  Richard W Ruhde
Institution:(1) Department of Horticulture, University of Wisconsin, Madison, Wisconsin, USA;(2) Plant Science Research Division, Agricultural Research Service, U.S. Department of Agriculture, Madison, Wisconsin, USA
Abstract:Summary The gene df (deformed flower), which is expressed as short anther in sensitive cytoplasm, has been incorporated into the trisomic series of S. chacoense Bitt. The cytoplasmic sensitivity and genotypes for the gene df were tested in the original trisomics and their parents. Two diploid parents were found to be homozygous dominant for Df, while one was homozygous recessive. All the parents and trisomics tested had resistant cytoplasm. Six trisomics were heterozygous for df, segregating with a 1:1 ratio when they were crossed to the homozygous testers (df s]dfdf). When the F1 trisomics (trisomics x Df r]dfdf) were crossed to the homozygous testers, nine of the 13 trisomics segregated with a 1:1 ratio, three with a 0:1 ratio (all deformed) and one trisomic (V1682.3) with a 2:1 ratio. All the parents of the trisomic V1682.3 were homozygous dominant for Df. This clone is believed to be trisomic for the gene df.Cooperative investigation of the Agricultural Research Service, U.S. Department of Agriculture and the Wisconsin Experiment Station, Madison.
Keywords:Solanum chacoense  deformed flower  genetics  trisomics  potato  ancuploid
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