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| 有机磷农药降解菌—阴沟肠杆菌的生物学特性 | |
| 引用本文： | 林抗美,官雪芳,马丽娜,王宗华,鲁国东,刘 波.有机磷农药降解菌—阴沟肠杆菌的生物学特性[J].中国农学通报,2008,24(9):382-386. |
| 作者姓名： | 林抗美 官雪芳 马丽娜 王宗华 鲁国东 刘 波 |
| 作者单位： | 1. 福建省农科院农业生物资源研究所,福州,350003 2. 福建省农科院农业工程技术研究所,福州,350003 3. 福建农林大学生命科学学院,福州,350002 |
| 摘 要： | 【研究目的】对有机磷农药降解菌阴沟肠杆菌的生物学特性进行研究，为研究其降解特性机理及应用提供理论依据。【方法】测定阴沟肠杆菌生长曲线、生理生化特征，筛选出最适生长培养基，测定不同温度、pH值、摇床振数、装液量下的生长状况，确定最适生长条件，测定对乐果、甲胺磷、敌敌畏、敌百虫的耐受力和利用情况。【结果】阴沟肠杆菌培养3h后进入对数生长期，可以利用分解大部分含碳化合物，在营养贫瘠的条件上生长，生长温度范围为10~43℃，生长pH值范围为6~12，对乐果、甲胺磷、敌敌畏、敌百虫的降解利用必须建立在一定的营养物质的基础上。【结论】该菌的繁殖速度快，在营养条件较贫瘠的条件下就可以生存，是一株生存能力很强的菌株，适合应用于土壤、水体环境中的有机磷农药的降解。 |
| 关 键 词： | 马铃薯 马铃薯 农杆菌 原生质体 菜豆几丁质酶基因 |
| 收稿时间： | 2008-05-21 |
| 修稿时间： | 2008-07-29 |
The Biological Characteristics of Organophosphorus Pesticide-degrading Bacterium, Enterobacter cloacae |
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| Lin Kangmei,Guan Xuefang,Ma Lin,Wang Zonghu,Lu Guodong,Liu Bo.The Biological Characteristics of Organophosphorus Pesticide-degrading Bacterium, Enterobacter cloacae[J].Chinese Agricultural Science Bulletin,2008,24(9):382-386. |
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| Authors: | Lin Kangmei Guan Xuefang Ma Lin Wang Zonghu Lu Guodong Liu Bo |
| Institution: | Institute of Agro-biological Resources, Fujian Academy of Agricultural Sciences, Fuzhou 350003; Institute of Agricultural Engineering Technology, Fujian Academy of Agricultural Sciences, Fuzhou 350003; Life Science College, Fujian Agriculture and Forestry University, Fuzhou 350002 |
| Abstract: | 【OBJECTIVE】The biological characteristics of organophosphorus pesticide-degrading bacterium, Enterobacter cloacae will be studied in this paper to explain the mechanism of degrading .【METHOD】The growth curve, physiological characteristics of E. cloacae were observed. Meanwhile, the suitable cultural medium, growth temperature, pH value, shaking speed, cultural volume were tested to determine the best cultural conditions for E. cloacae. And the endure and utilization abilities of E. cloacae to Dimethoate, Methamidophos, Dichlorvos and Trichlorfon were measured.【RESULTS】E. cloacae started logarithmic growth after 3 hours, and could utilize a great part of carbon-containing compounds and grow under nutrition-lacking condition. Its suitable growth temperature and pH were 10-43℃ and 6-12, respectively. Its degradation to Dimethoate, Methamidophos, Dichlorvos and Trichlorfon was base on the existence of certain nutrition materials.【CONCLUSION】E. cloacae had fast growth speed and could survive under nutrition-lacking condition, which meant that it was a bacterium suitable for degradation of organophosphorus pesticides in soil and water. |
| Keywords: | organophosphorus pesticidezz degrading bacteriumzz Enterobacter cloacaezz |
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