| 应用聚合酶链式反应鉴定新疆棉花落叶型黄萎病菌 |
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| 引用本文： | 张莉,段维军,李国英,宋蓓.应用聚合酶链式反应鉴定新疆棉花落叶型黄萎病菌[J].植物检疫,2004,18(5):266-268. |
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| 作者姓名： | 张莉 段维军 李国英 宋蓓 |
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| 作者单位： | 1. 西北农林科技大学植保学院,陕西杨陵,712100;新疆兵团绿洲生态农业重点实验室
2. 新疆兵团绿洲生态农业重点实验室 |
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| 摘 要： | 用一对棉花落叶型黄萎病菌的特异性引物D1和D2进行PCR扩增,对于落叶型黄萎病菌,该对引物可特异性地扩增产生一段550bp的产物,而非落叶型黄萎病菌则不能被扩增.供试的35个新疆黄萎菌系中,有3个菌系扩增出550bp大小的落叶型黄萎病菌特异性片段,表明目前新疆已存在落叶型黄萎病菌,用此技术可快速、准确地检疫和鉴定落叶型黄萎病菌.
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| 关 键 词： | 落叶型黄萎病菌 PCR扩增 鉴定 |
The identification of defoliatiny pathotype of Verticillium dahliae on cotton in Xinjiang by PCR |
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| Zhang Li ,Duan Weijun,Li Guoying,Song Pei.The identification of defoliatiny pathotype of Verticillium dahliae on cotton in Xinjiang by PCR[J].Plant Quarantine,2004,18(5):266-268. |
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| Authors: | Zhang Li Duan Weijun Li Guoying Song Pei |
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| Institution: | Zhang Li 1,2,Duan Weijun2,Li Guoying2,Song Pei2 |
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| Abstract: | A PCR fragment (550bp) from defoliating pathotype of Verticillium dahliaewas produced with a couple of specific primers, but not from nondefoliating pathotype of Verticillium dahliae. Among the 35 tested strains of Verticillium dahliaein Xinjiang, three of them can amplify the specific fragment (550bp) of defoliating pathotype of Verticillium dahliae.This result shows that defoliating pathotype of Verticillium dahliae have existed in Xinjiang, and this detecting technique is a rapid and accurate method for quarantine and identification of defoliating pathotype of Verticillium dahliae. |
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| Keywords: | defoliating pathotype of Verticillium dahliae PCR amplification identification |
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