| 猪流行性腹泻病毒ORF3和M基因的克隆与序列分析 |
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| 引用本文： | 王隆柏,吴学敏,车勇良,陈如敬,魏宏,刘玉涛,庄向生,周伦江.猪流行性腹泻病毒ORF3和M基因的克隆与序列分析[J].中国动物传染病学报,2012,20(3):67-72. |
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| 作者姓名： | 王隆柏 吴学敏 车勇良 陈如敬 魏宏 刘玉涛 庄向生 周伦江 |
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| 作者单位： | 福建省农业科学院畜牧兽医研究所福建省畜禽疫病防治工程技术研究中心,福州,350013 |
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| 基金项目： | 国家公益性行业(农业)科研专项(NYHYZX07-034) |
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| 摘 要： | 为了解福建省猪流行性腹泻病毒(Porcine epidemic diarrhea virus,PEDV)毒株ORF3和M基因变异情况,本研究从4个不同规模猪场发生仔猪“顽固性腹泻”疾病的病料中扩增到4株PEDV的ORF3和M基因,并进行了序列分析.结果表明:4株PEDV ORF3基因均含有675个碱基,编码224个氨基酸,与GenBank中登录的代表性PEDV核苷酸的同源性为89.7％～100.0％,氨基酸的同源性为94.7％～99.6％,其中FJPT毒株核苷酸的同源性与CV777 truncated毒株最低,仅为89.7％,FJNP毒株核苷酸的同源性与中国北方流行的CH ZKFG 11毒株最高,达100.0％.M基因含有681个碱基,编码226个氨基酸,与GenBank中登录的代表性PEDV核苷酸的同源性为94.8％～100.0％,氨基酸的同源性为94.8％～99.6％,其中FJPT毒株核苷酸的同源性与日本D89752毒株最低,仅为94.8％,FJNP毒株核苷酸的同源性与韩国CPF193、泰国毒株最高,达100.0％.4株PEDV毒株均与国内外流行强毒株的亲缘关系较近,与attenuate DR13弱毒株的亲缘关系较远.
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| 关 键 词： | 猪流行性腹泻病毒 ORF3基因 M基因 序列分析 |
CLONING AND SEQUENCE ANALYSIS OF ORF3 AND M GENE OF PORCINE EPIDEMIC DIARRHEA VIRUS |
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| WANG Long-bai,WU Xue-min,CHE Yong-liang,CHEN Ru-jing,WEI Hong,LIU Yu-tao, ZHUANG Xiang-sheng,ZHOU Lun-jiang.CLONING AND SEQUENCE ANALYSIS OF ORF3 AND M GENE OF PORCINE EPIDEMIC DIARRHEA VIRUS[J].Chinese Journal of Animal Infectious Diseases,2012,20(3):67-72. |
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| Authors: | WANG Long-bai WU Xue-min CHE Yong-liang CHEN Ru-jing WEI Hong LIU Yu-tao ZHUANG Xiang-sheng ZHOU Lun-jiang |
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| Institution: | (Institute of Animal Husbandry and Veterinary Medicine,Fujian Academy of Agriculture Sciences,Fujian Animal Disease Control Technology Development Center,Fuzhou 350013,China) |
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| Abstract: | The sequences of four Porcine epidemic diarrhea viruses(PEDVs) were amplified from clinical samples on four pig farms with refractory diarrhea in Fujian.The ORF3 and M gene of these PEDVs were sequenced and compared with those deposited in GenBank.The ORF3 consisted of 675 nucleotides,encoding a 224-amino acid protein.These four PDEV strains shared 94.7%-99.6% amino acid identities with the PEDV strains in GenBank.The FJPT strain shared the lowest nucleotide homology at 89.7% with CV777 truncated strain while FJNP strain shared the highest nucleotide homology at 100.0% to CH ZKFG 11.The M gene consisted of 681 nucleotides,encoding a 226-amino acid protein.The M gene of these four PDEV strains had 94.8%-99.6% amino acid homology with the PEDV strains in GenBank.The FJPT strain shared the lowest nucleotide homology at 94.8% with D89752 strain while FJNP strain had the highest nucleotide homology at 100.0% with CPF193.Phylogenetic analysis indicated that the four PEDV strains were close to velogenic strain and distant with the attenuated DR13 strain. |
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| Keywords: | Porcine epidemic diarrhea virus ORF3 gene M gene sequence analysis |
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