| 产单核细胞李斯特菌的PFGE分子分型分析 |
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| 引用本文： | 颜卫,焦新安,潘志明,黄金林,殷月兰.产单核细胞李斯特菌的PFGE分子分型分析[J].中国兽医学报,2012,32(8):1154-1157. |
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| 作者姓名： | 颜卫 焦新安 潘志明 黄金林 殷月兰 |
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| 作者单位： | 1. 江苏畜牧兽医职业技术学院,江苏泰州,225300
2. 扬州大学江苏省人兽共患病学重点实验室,江苏扬州,225009 |
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| 摘 要： | 对50株不同来源的产单核细胞李斯特菌（Lm）进行PFGE分型,比较食源、环境、人源菌株之间的相似性。结果显示,同一市场内,环境污水分离株和新鲜食品分离株表现出较高同源性,新鲜食品分离株与即食性（RTE）食品分离株相似性很高,临床脑膜炎病人的1株Lm与RTE食品分离株在进化上显示出了很近的亲缘关系。50株Lm的分子分型分析提示了菌株从市场环境污水-新鲜食品-RTE食品-人的传播模式,为食源性疾病溯源追踪提供了新的思路。
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| 关 键 词： | 产单核细胞李斯特菌 脉冲场凝胶电泳 分子分型 |
Molecular stubtyping of Listeria monocytogenes by pulsed-field gel electrophorsis |
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| YAN Wei,JIAO Xin-an,PAN Zhi-ming,HUANG Jin-lin,YIN Yue-lan.Molecular stubtyping of Listeria monocytogenes by pulsed-field gel electrophorsis[J].Chinese Journal of Veterinary Science,2012,32(8):1154-1157. |
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| Authors: | YAN Wei JIAO Xin-an PAN Zhi-ming HUANG Jin-lin YIN Yue-lan |
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| Institution: | 1.Jiangsu Animal Husbandry & Veterinary College,Taizhou,Jiangsu 225300,China;2.Jiangsu Key Laboratory of Zoonosis,Yangzhou University,Yangzhou,Jiangsu 225009,China) |
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| Abstract: | Using the technique of pulsed-field gel electrophoresis(PFGE) established a relationship of Listeria monocytogenes(Lm) by comparing the genome of strains.This method provided a useful tool for epidemiological investigation.Subtyping 50 strains of Lm which was isolated from food,environment and patients with clinical meningitis.The results showed that in the same market,L.monocytogenes strains isolated from environmental sewages are very similar to those isolated from resh food products.The high similarity also exist in strains from resh food products and RTE food.One clinical isolate had a close genetic relationship with strain from RTE food.The results suggest a mode of transmission of strains from the market environmental sewages-fresh food-the RTE food-people.This study provide a new way for tracing the origin of foodborne disease. |
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| Keywords: | Listeria monocytogenes pulsed-field gel electrophoresis molecular stubtype |
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