| 1株产胆红素氧化酶新菌株的分离与鉴定研究 |
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| 引用本文： | 黄世臣,赵敏.1株产胆红素氧化酶新菌株的分离与鉴定研究[J].安徽农业科学,2014(12):3486-3490. |
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| 作者姓名： | 黄世臣 赵敏 |
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| 作者单位： | 东北林业大学生命科学学;延边大学农学院; |
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| 基金项目： | 中国国家林业厅984项目(2012-4-03);自然科学基金(31170553,30671702,30170775) |
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| 摘 要： | 目的]分离与鉴定1株产胆红素氧化酶的新菌株.方法]利用常规的微生物分离培养技术,从玉米叶片的病斑中分离出一株产胆红索氧化酶（bilirubin oxidase,BOD）的丝状真菌.结果]通过形态学、生理学和rDNA-ITS（登录号为JN251106）序列分析,该菌株被鉴定为Bipolaris australiensis,命名为B.australiensis HD-1,属子囊菌门格孢腔菌目格孢腔菌科平脐蠕孢属澳大利亚平脐蠕孢霉（B.amtraliensis）,亦称澳洲双孢霉,是1株没有被报道过的具有产BOD的新产生菌;在不合任何诱导剂的发酵液中28℃,150 r/min,培养6d,其酶活可达为1 000 U/L.结论]该方法成功分离出1株产胆红素氧化酶的新菌株,为BOD的产生菌提供了新的微生物来源.
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| 关 键 词： | 胆红素氧化酶 Bipolaris australiensis 分离鉴定 |
Isolation and Identification of Novel Producing Bilirubin Oxidase Microorgansims |
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| HUANG Shi-chen,ZHAO Min.Isolation and Identification of Novel Producing Bilirubin Oxidase Microorgansims[J].Journal of Anhui Agricultural Sciences,2014(12):3486-3490. |
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| Authors: | HUANG Shi-chen ZHAO Min |
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| Institution: | 1. College of Life Science, Northeast Forestry University, Harbin, Heilongjiang 1500d0; 2. Agricuttare School, Yanbiao University Yanji, Jilin 133002) |
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| Abstract: | Objective ] To isolate and identify a novel strain for producing bilirubin oxidase. Method ] The strain producing BOD was isolated from diseased leaves of maize by means of conventional microbiological separation techniques. Result] The strain was identified as Bipolaris au- straliensis, named B. australiensis HD-l by morphology, physiology and rDNA -ITS （accession number JN251106） sequence analysis, which belong to phylum ascomycota, class dot,hideomycetes, order pleosporales, genus bipolaris and Bipolaris australiensis, having enzyme activity 1 000 U/L under conditions that 28 ℃, 150 rpm for 6 d without any inducers of primary culture medium broth. Conclusion] It was a novel strain as so far. A new microbial source producing BOD was found. |
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| Keywords: | Bilirubin oxidase Bipolaris australiensis Isolation and identification |
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