| 梅花鹿鹿茸表皮生长因子基因原核表达载体的构建及鉴定 |
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| 引用本文： | 苏凤艳,宗颖,何忠梅.梅花鹿鹿茸表皮生长因子基因原核表达载体的构建及鉴定[J].经济动物学报,2012,16(3):129-132. |
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| 作者姓名： | 苏凤艳 宗颖 何忠梅 |
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| 作者单位： | 吉林农业大学中药材学院,长春,130118 |
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| 基金项目： | 吉林省教育厅项目[2009第(66)号];吉林省自然科学基金项目(201115194) |
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| 摘 要： | 通过T-A克隆技术,成功地构建了克隆载体pMD-18T-EGF,用EcoRⅠ和HindⅢ双酶切克隆质粒pMD-18T-EGF后,回收EGF基因,并将此基因定向克隆至相同双酶切回收后的pET32a原核表达载体中,获得重组质粒pET32a-EGF。经限制性内切酶分析和PCR鉴定,结果表明:成功地构建了原核重组质粒。
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| 关 键 词： | 梅花鹿 鹿茸 表皮生长因子 原核表达载体 |
Construction and Identification of Prokaryotic Expression Plasmid for Epidermal Growth Factor Gene of Sika Deer |
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| SU Feng-yan,ZONG Ying,HE Zhong-mei.Construction and Identification of Prokaryotic Expression Plasmid for Epidermal Growth Factor Gene of Sika Deer[J].Journal of Economic Animal,2012,16(3):129-132. |
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| Authors: | SU Feng-yan ZONG Ying HE Zhong-mei |
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| Institution: | (Chinese Medicinal Materials College,Jilin Agricultural University,Changchun 130118,China) |
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| Abstract: | The clone vector pMD-18T-EGF was constructed successfully by T-A clone technique and was digested with EcoRⅠand HindⅢ,and the prokaryotic expression vector pET32a was also cut,then the gene was cloned into the vector.Finally a recombinant plasmid named pET32a-EGF was obtained,and identified by PCR,restriction enzyme analysis and sequencing. |
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| Keywords: | sika deer antler epidermal growth factor prokaryotic expression vector |
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