| 60Co-γ射线辐照对澳洲坚果花粉萌发的影响 |
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| 引用本文： | 柳觐 孔广红 倪书邦 贺熙勇 陶,丽 陈丽兰 张海文.60Co-γ射线辐照对澳洲坚果花粉萌发的影响[J].中国农学通报,2014,30(4):132-136. |
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| 作者姓名： | 柳觐 孔广红 倪书邦 贺熙勇 陶 丽 陈丽兰 张海文 |
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| 作者单位： | 1. 云南省热带作物科学研究所2. |
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| 基金项目： | 澳洲坚果新品种（系）选育;澳洲坚果突变体新种质的创制;澳洲坚果种质资源保护;澳洲坚果激素促脱高效采收方式示范推广 |
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| 摘 要： | 为探明不同剂量^60Co-γ射线对不同品种澳洲坚果花粉萌发的影响；采用不同剂量^60Co-γ射线对澳洲坚果品种‘HAES900’和‘HAEs951’的新鲜花粉进行辐照处理，并对辐照的后花粉活力和花粉管形态进行观测；经低剂量^60Co-γ射线辐照后，‘HAES900’的花粉离体萌发率随着剂量的增加呈现出增高趋势，而‘HAES951’的萌发率呈现出先升高再降低的趋势，但差异均未达到显著水平；经高剂量^60Co-γ射线辐照后，‘HAES900’的花粉离体萌发率随着剂量的增加先增高然后显著降低，而‘HAES951’的萌发率呈现出极显著的下降趋势；高剂量辐照后，同一处理内花粉管长度变异幅度增大，部分花粉管出现扭曲或顶端膨大等形态变异；‘HAES900’和‘HAES951’的致死剂量均为600Gy，半致死剂量分别为480Gy和360Gy。本研究发现，低剂量辐照可促进澳洲坚果的花粉离体萌发，而高剂量的辐照则会抑制花粉萌发；‘HAES951’的花粉对^60Co-γ射线辐照更为敏感。
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| 关 键 词： | 澳洲坚果 花粉萌发 ^60Co-γ射线 剂量 |
| 收稿时间： | 2013/9/16 0:00:00 |
| 修稿时间： | 2013/10/30 0:00:00 |
The Effects of ^60Co-γ Ray Irradiation on Germination of MacadamiaPollen |
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| Liu Jin,Kong Guanghong,Ni Shubang,He Xiyong,Tao Li,Chen Lilan,Zhang Haiwen.The Effects of ^60Co-γ Ray Irradiation on Germination of MacadamiaPollen[J].Chinese Agricultural Science Bulletin,2014,30(4):132-136. |
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| Authors: | Liu Jin Kong Guanghong Ni Shubang He Xiyong Tao Li Chen Lilan Zhang Haiwen |
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| Institution: | (Yunnan Institute of Tropical Crops, Jinghong Yunnan 666100) |
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| Abstract: | In order to explore the effects of ^60Co-γ ray irradiation on the pollen germination of different macadamia cultivars, the fresh pollen of macadamia cultivar ＇ HAES900＇ and ＇ HAES951＇ were irradiated by ^60Co-γ ray at different dose with the dose rate of 10 Gy/h, the pollen viability and morphology were observed. The results indicated that the in vitro pollen germination rate of ＇HAES900＇ increased with dose increasing after low dose irradiation and the pollen germination rate of ＇ HAES951＇ increased firstly and then decreased. After irradiation at high dose, the in vitro pollen germination rate of ＇HAES900＇ increased firstly and then significantly decreased, and ＇HAES951＇ decreased significantly. The variation of pollen tube length in the same treatment was larger after irradiation of ^60Co-γ ray at high dose; some pollen tubes appeared distorted or intumescent at the top. The lethal dose of both ＇HAES900＇ and ＇HAES951＇ were 600 Gy, but the LDso of ＇ HAES900＇ and ＇HAES951＇ was 480 Gy and 360 Gy respectively. The study found that irradiation of ^60Co-γ ray at low dose could promote the macadamia pollen germination in vitro and at high dose could inhibit the pollen germination; the cuhivar ＇ HAES951＇ was more sensitive to ^60Co-γ ray irradiation. |
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| Keywords: | Dose |
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