| 高通量测序技术在土壤微生物多样性研究中的应用 |
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| 引用本文： | 楼骏,柳勇,李延.高通量测序技术在土壤微生物多样性研究中的应用[J].中国农学通报,2014,30(15):256-260. |
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| 作者姓名： | 楼骏 柳勇 李延 |
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| 作者单位： | 1. 福建农林大学资源与环境学院2. |
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| 基金项目： | 钙及钙受体在黄瓜抗自毒胁迫中的作用机理 |
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| 摘 要： | 为了阐明高通量测序技术在土壤微生物多样性研究中的应用前景，归纳介绍了Solexa、454和Ion Torrent等常用高通量测序技术的原理和优点，综述了近年高通量测序技术在土壤微生物物种多样性、结构多样性、功能多样性和遗传多样性四个方面应用的研究进展，总结了高通量测序技术应用中存在的问题，并分析展望了其在土壤微生物多样性研究中的发展趋势。
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| 关 键 词： | 稻瘟病 稻瘟病 化学防治 抗性品种 生物防治 栽培措施 |
| 收稿时间： | 2013/9/24 0:00:00 |
| 修稿时间： | 2013/10/28 0:00:00 |
Review of High-throughput Sequencing Techniques in Studies of Soil Microbial Diversity |
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| Li Yan.Review of High-throughput Sequencing Techniques in Studies of Soil Microbial Diversity[J].Chinese Agricultural Science Bulletin,2014,30(15):256-260. |
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| Authors: | Li Yan |
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| Abstract: | In order to illuminate the application prospects of high-throughput sequencing techniques in soil microbial diversity, this article gave a summarization and introduction of principles and advantages of common high-throughput sequencing techniques, including Solexa, 454 and Ion Torrent. The latest research advances of high- throughput sequencing techniques applications were reviewed in soil microbial species diversity, structures diversity, functions diversity and genes diversity. Finally, the application problems and the development tendency of high- throughput sequencing techniques used in soil microbial studies were summarized and discussed. |
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| Keywords: | application |
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