| 黄花矶松组织培养及培养基筛选研究 |
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| 引用本文： | 王方琳,崔建国,尉秋实,张莹花,柴成武.黄花矶松组织培养及培养基筛选研究[J].中国农学通报,2014,30(13):45-50. |
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| 作者姓名： | 王方琳 崔建国 尉秋实 张莹花 柴成武 |
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| 作者单位： | 1. 甘肃省治沙研究所2. 3. 甘肃民勤荒漠草地生态系统国家野外科学观测研究站/甘肃省治沙研究所4. 甘肃省荒漠化与风沙灾害防治重点实验室-省部共建国家重点实验室培育基地&甘肃省治沙研究所5. 甘肃省荒漠化防治与风沙灾害国家重点试验室/甘肃省治沙研究所 |
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| 基金项目： | 国家科技计划（2012）“祁连山水源涵养林生态系统保育技术试验示范”（BAD16B00）; 国家自然科学基金“沙区粘土质夹层土壤水分分布与植物的生态响应”（31100519） |
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| 摘 要： | 试验选用种子培养的黄花矶松无菌苗的子叶、茎段、下胚轴作为外植体材料,研究不同外植体的离体培养技术及其适宜的培养基。结果表明,生长素2,4-D对不定芽诱导具有明显的促进作用,在其浓度为1.5 mg/L时诱导率最高,子叶是诱导不定芽的良好外植体,最适培养基为MS＋2,4-D 1.5 mg/L＋6-BA 2.0 mg/L＋NAA 1.0 mg/L。黄花矶松的最适增殖培养基为MS＋6-BA 1.0 mg/L＋NAA 1.0 mg/L,而且是以丛生芽的方式进行增殖的;最适生根培养基是1/2 MS＋KT 1.0 mg/L＋IBA1.0 mg/L。
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| 关 键 词： | 黄花矶松 组织培养 培养基 愈伤组织 |
| 收稿时间： | 2013/11/10 0:00:00 |
| 修稿时间： | 2014/1/10 0:00:00 |
Research on Tissue Culture and Medium of Limoniumau-reum |
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| Wang Fanglin,Cui Jianguo,Yu Qiushi,Zhang Yinghua,Chai Chengwu.Research on Tissue Culture and Medium of Limoniumau-reum[J].Chinese Agricultural Science Bulletin,2014,30(13):45-50. |
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| Authors: | Wang Fanglin Cui Jianguo Yu Qiushi Zhang Yinghua Chai Chengwu |
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| Institution: | (The State Key Laboratory of Desertification Combating Prevention and Sandstorm Disaster of Gansu Province/ Gansu Desert Control Research Institute, Wuwei Gansu 733000) |
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| Abstract: | Abstract: Experiment chooses seed development Limoniumau-reum no vaccine hypocotyl cotyledon stem section as explant materials ,study the culture of different explant technique, the results show that: the suitable medium auxin 2, 4-d on adventitious bud induction has an obvious role in promoting, when its concentration is 1.5 mg/L induction rate is highest, cotyledon is good explant of adventitious bud induction, the optimal culture medium for MS+ 2,4-D 1.5 mg/L+ 6-BA 2.0 mg/L+ NAA 1.0 mg/L Limoniumau-reum optimal proliferation medium was MS+6-BA 1.0 mg/L+ NAA 1.0 mg/L, and took the form of multiple shoot clumps proliferation; The optimum rooting medium was 1/2 MS+ KT 1.0 mg/L+ IBA 1.0 mg/L. |
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| Keywords: | callus tissue |
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