| 一种快速提取甘薯基因组DNA方案的建立 |
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| 引用本文： | 吴秋云,罗文彬,邱永祥,蔡南通.一种快速提取甘薯基因组DNA方案的建立[J].山西农业大学学报(自然科学版),2006,26(2):119-120,124. |
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| 作者姓名： | 吴秋云 罗文彬 邱永祥 蔡南通 |
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| 作者单位： | 福建省农业科学院,作物研究所,福建,福州,350013 |
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| 基金项目： | 福建省科技重大专项(2004NZ01) |
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| 摘 要： | 建立了一种小量快速提取甘薯基因组DNA的方法。用该法以甘薯幼叶为实验材料,用小量快速法提取甘薯基因组DNA。提取的DNA经酶切、琼脂糖凝胶电泳检测,结果表明DNA质量较好,所提取的DNA可用于RAPD、AFLP等技术。此方法具有提取速度快、利于规模化提取、DNA质量好和经济实用等优点,为甘薯及其它相关植物基因组DNA的提取提供了一种快速、简便的途径。
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| 关 键 词： | 甘薯 基因组DNA提取 快速法 |
| 文章编号： | 1671-8150（2006）02-0119-02 |
| 收稿时间： | 2005-09-09 |
| 修稿时间： | 2005-09-09 |
A Method for Rapid Isolation of Genomic DNA in Sweetpotato on Small Scale |
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| WU Qiu-yun et al..A Method for Rapid Isolation of Genomic DNA in Sweetpotato on Small Scale[J].Journal of Shanxi Agricultural University,2006,26(2):119-120,124. |
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| Authors: | WU Qiu-yun |
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| Institution: | Crop Institute of Fujian Agriculture Academy of Science, Fuzhou Fujian 350013, China |
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| Abstract: | A new method for rapid isolation of genomic DNA on small scale in sweetpotato was established.Genomic DNA from young leaves of sweetpotato was extracted by a new small scale and rapid isolating method.The extracted DNA by the new method was in a good quality,and could be used in RAPD,SSR and AFLP.The method displayed many fine characteristics such as high efficiency,good quality,practicality and so on.It is a good way for extracting genomic DNA from sweetpotato and other plants. |
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| Keywords: | Sweetpotato Genomic DNA isolation Small scale and rapid isolation |
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