| 湖南省稻瘟病菌无毒基因鉴定 |
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| 引用本文： | 刘翔,任佐华,陈娟芳,毛锐,戴良英,刘二明.湖南省稻瘟病菌无毒基因鉴定[J].南方农业学报,2016(9):1500-1505. |
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| 作者姓名： | 刘翔 任佐华 陈娟芳 毛锐 戴良英 刘二明 |
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| 作者单位： | 湖南农业大学 植物保护学院/植物病虫害生物学与防控湖南省重点实室/南方粮油作物协同创新中心,长沙,410128 |
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| 基金项目： | 公益性行业(农业)科研专项项目(201203014)；湖南省自然科学基金青年项目(12JJ4028) |
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| 摘 要： | 【目的】了解湖南省稻瘟病菌无毒基因组成,为培育水稻新品种及合理布局抗稻瘟病品种提供参考依据。【方法】采用2014年分离自湖南桃江病圃丽江新团黑谷(LTH)上的120个稻瘟病菌单孢菌株室内离体针刺接种于24个已知抗瘟基因的水稻近等基因系(NILs)上,对供试菌株进行无毒基因鉴定。【结果】120个稻瘟病菌含有全部24个无毒基因,在24个无毒基因中,出现频率高于40.00%的有Avr-Pita和Avr-Pish;出现频率在30.00%~40.00%的无毒基因有Avr-Piz、Avr-Piz5、Avr-Pi3和Avr-Pi9;其余无毒基因出现频率均较低,特别是Avr-Piks、Avr-Pikp、Avr-Pib、Avr-Pi12和Avr-Pi11出现频率均低于10.00%。【结论】含抗性基因Pi-ta和Pi-sh的水稻品种可在湖南省推广利用;含抗性基因Pi-z、Pi-z5、Pi-3和Pi-9的水稻品种在湖南推广时应慎重考虑;含抗性基因Pi-ks、Pi-kp、Pi-b、Pi-12和Pi-11的水稻品种不宜在湖南省推广利用。
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| 关 键 词： | 稻瘟病菌 近等基因系 离体接种 无毒基因 湖南省 |
Identification of avirulent genes in Magnaporthe oryzae in Hunan province |
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| Abstract: | Objective]Composition of avirulent genes types of Magnaporthe oryzae in Hunan province were studied, in order to provide reference basis for breeding of new rice cultivars and rational distribution of blast -resistant cultivars.Method]A total of 120 M. oryzae monadic isolates were isolated from rice susceptible cultivars (Lijiangxintuanheigu, LTH) in Hunan Taojiang nurseries in 2014. Then, 24 near-isogenic lines (NILs) with known blast-resistance gene was in vitro inoculated artificially with above isolates by acupuncture, and the avirulent genes of tested isolates were identified.Result]The above 120 M. oryzae isolates all carried 24 avirulent genes, among 24 avirulent genes, occurrence frequencies of Avr-Pita and Avr-Pish were more than 40.00%, while occurrence frequencies of Avr-Piz, Avr-Piz5, Avr-Pi3 and Avr-Pi9 ranged from 30.00% to 40.00%, occurrence frequencies of the rest were lower relatively, especially Avr-Piks, Avr-Pikp, Avr-Pib, Avr-Pi12 and Avr-Pi11 with frequency of less than 10.00%. Conclusion]The rice cultivars carrying resistant genes Pi-ta and Pi-sh sould be generalized in Hunan province; the rice varieties cultivars carrying resistant genes Pi-z, Pi-z5, Pi-3 and Pi-9 should be considered carefully in their utilization and extension in Hunan province; the rice cultivars carrying blast-resistant genes Pi-ks, Pi-kp, Pi-b, Pi-12 and Pi-11 are not suitable for utilization and extension in Hunan province. |
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| Keywords: | Magnaporthe oryzae near-isogenic lines(NILs) in vitro inoculation avirulent gene Hunan province |
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