| 大穗型水稻穗部性状的QTL定位 |
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| 引用本文： | 汪欲鹏,段里成,龙启樟,徐林典,武志峰,万建林,石庆华,潘晓华,吴自明.大穗型水稻穗部性状的QTL定位[J].南方农业学报,2016(9):1445-1449. |
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| 作者姓名： | 汪欲鹏 段里成 龙启樟 徐林典 武志峰 万建林 石庆华 潘晓华 吴自明 |
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| 作者单位： | 1. 作物生理生态与遗传育种教育部重点实验室/江西省作物生理生态与遗传育种重点实验室/双季稻现代化生产协同创新中心/江西农业大学,南昌,330045;2. 江西省超级水稻研究发展中心,南昌,330200;3. 贵溪市农业技术推广服务中心,江西 鹰潭,335400 |
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| 基金项目： | 国家自然科学基金项目(31560350)；国家重点研发计划项目(2016YFD0101105)；江西省科技支撑计划项目(20132BBF60004) |
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| 摘 要： | 【目的】对大穗型水稻穗部性状的数量性状基因座(QTL)进行定位,为水稻超高产育种提供优质的种质材料。【方法】利用95个在双亲具有明显多态性的分子标记和213个由大穗型水稻材料1p1与9311构建的F_2群体单株,采用完备区间加性模型作图法(ICIM-ADD)对穗长、每穗粒数和着粒密度3个穗部性状的QTL进行检测。【结果】共检测到5个穗部性状QTL,其中穗长QTL 1个,每穗粒数QTL 2个,着粒密度QTL 2个,分布于第3、4、6、10和11号染色体上。检测到的QTL LOD介于2.63~2.91,表型贡献率为7.42%~17.72%,贡献率大于10.00%的主效QTL有2个(qSSD-3-1和qPL-11-1),分别有2个和3个QTL的增效等位基因来源于大穗1p1和9311。【结论】定位得到的主效QTL qSDD-3-1和qPL-11-1可用于分子标记辅助选择育种,新的穗长QTL qPL-11-1可用于精细定位和克隆。
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| 关 键 词： | 水稻 QTL定位 穗部性状 |
QTL analysis of panicle traits in large panicle rice |
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| Abstract: | Objective]The present study was conducted to locate QTLs of panicle traits in large panicle rice, in order to provide high-quality germplasm resources in breeding programs of super high-yield rice. Method]A F2 population in-cluding 213 plants derived from hybrid of large panicle materials lp1 and 9311, and 95 molecular markers which had ob-vious polymorphism in bi-parents were used. Then inclusive composite interval mapping(ICIM) method were utilized to detect QTLs of panicle length(PL), spikelet number per panicle(SPP), and seed setting density(SSD) in this population.Result]A total of 5 related QTLs were detected on chromosome 3, 4, 6, 10 and 11, including 1 QTLs for PL, 2 Ma-jor QTLs for SPP and 2 QTLs for SSD. The LOD scores of QTLs ranged from 2.63 to 2.91, and the phenotypic variation explained(PVE) ranged from 7.42% to 17.72%, and there were 2 QTLs(qSSD-3-1 and qPL-11-1) whose PVE were more than 10.00%. 2 QTLs and 3 QTLs source of positive allele were lp1 and 9311, respectively. Conclusion]The detected qSSD-3-1 and qPL-11-1 are used in marker-assisted selection breeding, and qPL-11-1, which is one new QTL of PL, can be used in fine mapping and clone. |
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| Keywords: | rice QTL mapping panicle trait |
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