| 利用细胞工程技术培育玉米耐盐自交系及单交种 |
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| 引用本文： | 张可炜,王先艳,王灵芝,张举仁,许方佐,徐立华,周柱华.利用细胞工程技术培育玉米耐盐自交系及单交种[J].山东农业科学,2001(6):15-17,24. |
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| 作者姓名： | 张可炜 王先艳 王灵芝 张举仁 许方佐 徐立华 周柱华 |
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| 作者单位： | 1. 山东大学生命科学学院,
2. 山东省农业科学院原子能农业应用研究所, |
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| 基金项目： | 本文为山东省教委"利用多种生物技术创造玉米育种新材料"的部分研究内容. |
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| 摘 要： | 以玉米骨干自交系黄早 4、齐 319和 8112的幼胚为外植体 ,用含有 10g/LNaCl的培养基筛选获取耐盐愈伤组织。耐盐愈伤组织分化出幼苗 ,自交结实。子一代通过水培进行耐盐性测定 ,选择耐盐植株 ,对其后代继续进行耐盐性鉴定、筛选 ,获得耐盐突变体。以农艺性状稳定和耐盐性稳定的自交系为材料 ,组配出耐盐杂交种N2 - 1×N1- 4A和 8112 - 1×N1- 6A等杂交组合。盐碱地试验表明 ,这些组合适合于在中度盐碱地种植 ,且在生长后期可以用 5 0 %海水灌溉
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| 关 键 词： | 耐盐愈伤组织 耐盐自交系 玉米 |
| 文章编号： | 1001-4942(2001)06-0015-03 |
Breeding of maize inbred lines and hybrids with the trait of salt-tolerance via cellular engineering |
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| Abstract: | Embryogenic calli were induced from the immature embryos of maize elite maize inbred lines of Huangzao 4,Qi 319 and 8112 NaCl-tolerant calli were selected by subculture on the medium containing NaCl 10g/L Then plantlets were regenerated from the NaCl-tolerant calli After transplantation the plants grew up and self-crossed The salt tolerance of the progeny was examined by water culture or sand culture and NaCl-tolerant plants were selected, then self-lines with good agronomic characteristics were obtained Salt-tolerant hybrids such as N2-1×N1-4A and 8112-1×N1-6A performed very well in middle saline soil |
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| Keywords: | NaCl-tolerant calli salt-tolerant inbred lines maize |
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