| 鸡志贺氏菌多价凝集抗原及其标准血清的应用 |
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| 引用本文： | 宋海霞,李炜,蒋大伟,许兰菊,李凯,潘国民,贾文科.鸡志贺氏菌多价凝集抗原及其标准血清的应用[J].河南农业科学,2009(11). |
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| 作者姓名： | 宋海霞 李炜 蒋大伟 许兰菊 李凯 潘国民 贾文科 |
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| 作者单位： | 河南农业大学牧医工程学院,河南,郑州,450002 |
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| 基金项目： | 国家自然科学基金项目，河南省重点科技攻关项目 |
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| 摘 要： | 利用研制的鸡志贺氏菌多价凝集抗原及其标准血清,对信阳地区18家鸡场的351份鸡待检血清进行了血清抗体检测。结果显示:351份被检血清中,鸡鲍氏和痢疾志贺氏菌混合感染的抗体阳性率达8.33%(1/12)～73.91%(17/23),平均抗体阳性率为44.16%(155/351)。通过与鸡志贺氏菌单价凝集抗原的检测结果相比较,发现其吻合率高达98.58%(346/351),说明鸡志贺氏菌多价凝集抗原及其标准血清用于血样的检测,具有高度的特异性,准确可靠、方法简便快速。
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| 关 键 词： | 鸡志贺氏菌多价凝集抗原 标准血清 血清学调查 抗体阳性率 |
The Application of Chicken Shigellae Polyvalent Antigen and Standard Serum |
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| SONG Hai-xia,LI Wei,JIANG Da-wei,XU Lan-ju,LI Kai,PAN Guo-min,JIA Wen-ke.The Application of Chicken Shigellae Polyvalent Antigen and Standard Serum[J].Journal of Henan Agricultural Sciences,2009(11). |
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| Authors: | SONG Hai-xia LI Wei JIANG Da-wei XU Lan-ju LI Kai PAN Guo-min JIA Wen-ke |
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| Abstract: | In this study,chicken polyvalent antigen and its standard serum are used for serum antibody detection of 351 chicken serum which from the 18 chicken farms of Xinyang.The results showed that:the mixed infection of antibody-positive rate of S.boydii and the S.dysenteriae is 8.33%(1/12)~73.91%(17/23),the average antibody-positive rate is 44.16%(155/351).Compared with the test result of Shigella monovalent antigen,the rate of anastomosis up to 98.58%(346/351),which shows that chicken Shigellae polyvalent antigen and its standard serum have high specificity and great accuracy,and the method is simple and fast.So the diagnostic reagents and method are perfect for basic veterinary departments and chicken house to test the infection of chicken Shigellae,having great significance and application and dissemination value. |
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| Keywords: | Polyvalent antigen of chicken Shigellae Standard serum Serological survey Antibody positiveness |
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