Development of a complete set of disomic rape-radish chromosome-addition lines |
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| Authors: | H Budahn O Schrader H Peterka |
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| Institution: | (1) Federal Centre for Breeding Research on Cultivated Plants, Institute of Horticultural Crops, Neuer Weg 22/23, 06484 Quedlinburg, Germany |
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| Abstract: | After crosses of multiple rape-radish chromosome additions with rape, nine different monosomic additions (2n = 4x = 38 + 1)
of individual radish chromosomes, a–i, were isolated, having the genomic background of winter oil seed rape and radish cytoplasm.
Extra chromosomes were identified with RAPD markers, specific for individual radish chromosomes. All radish-plasmic monosomic
additions, except that of chromosome f, had low seed production due to pistilloid stamens. Rape cytoplasm was substituted
by crossing the monosomic additions as pollinators to rape followed by reselection of the radish chromosome additions. The
monosomic additions in rape cytoplasm showed normal male fertility. Average transmission rates of radish chromosomes via egg
cells and pollen cells were 37% and 27%, respectively. Lowest male transmission was found for chromosomes e and f with 0.04
and 0.01. Monosomic additions were self-pollinated to produce disomic additions. Monosomic and disomic addition progenies
were discriminated by a prescreening with quantitative double primer (dp) RAPDs and cytological confirmation of preselected
candidates by FISH analysis using a Raphanus-specific probe. Usability of this complete set of nine disomic rape-radish additions (2n = 4x = 38 + 2) was discussed. |
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| Keywords: | Brassica napus Raphanus sativus FISH Chromosome-specific marker Interspecific hybridization Monosomic alien addition line |
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