| Analysis of the Offspring Sex Ratio of Chicken by Using Molecular Sexing |
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| 作者姓名： | FENG Yan-ping GONG Yan-zhang Nabeel Ahmed Affara PENG Xiu-li YUAN Jin-feng ZHAO Rui-Xia Mohammed Yusuf Osman Jeffer ZHANG Shu-jun |
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| 作者单位： | [1]College of Animal Science, Huazhong Agricultural University, Wuhan 430070, P.R.China [2]Department of Pathology, Cambridge University, Cambridge CB1 2QP, UK |
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| 基金项目： | 湖北省武汉市科研项目;国家科技计划 |
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| 摘 要： | The overall sex ratio of offspring （dead embryos and hatch chicks） from all the fertilized eggs of 140 hens collected for 30 days was studied using duplex PCR of certain fragments of sex chromosomes. Additional 894 dead embryos over a period of 21 days of incubation were also investigated to verify the sex ratio of the dead embryos. The sex of the early dead embryos was identified using this molecular sexing technique. The sex ratio of the hatch chicks and the total offspring of the hens investigated in this experiment did not differ from the expected sex ratio （i.e., 1：1）., However, the number of female dead embryos was significantly more than that of males. The data indicated that the different physiologic function of males and females contributed to female-biased mortality during incubation. It was also found by further analysis that the sex ratios of the offspring of some hens were significantly biased to female or male over the period investigated, which suggested that the sex ratio of offspring might be influenced by the maternal condition to some degrees.
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| 关 键 词： | 鸡 分子控制性别 存活率 生物技术 |
| 收稿时间： | 2005-11-13 |
| 修稿时间： | 2006-04-29 |
Analysis of the Offspring Sex Ratio of Chicken by Using Molecular Sexing |
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| FENG Yan-ping GONG Yan-zhang Nabeel Ahmed Affara PENG Xiu-li YUAN Jin-feng ZHAO Rui-Xia Mohammed Yusuf Osman Jeffer ZHANG Shu-jun.Analysis of the Offspring Sex Ratio of Chicken by Using Molecular Sexing[J].Agricultural Sciences in China,2006,5(7):545-549. |
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| Authors: | FENG Yan-ping GONG Yan-zhang Nabeel Ahmed Affara PENG Xiu-li YUAN Jin-feng ZHAO Rui-xia Mohammed Yusuf Osman Jeffer ZHANG Shu-jun |
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| Institution: | 1. College of Animal Science, Huazhong Agricultural University, Wuhan 430070, P.R. China
2. Department of Pathology, Cambridge University, Cambridge CB1 2QP, UK |
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| Abstract: | The overall sex ratio of offspring (dead embryos and hatch chicks) from all the fertilized eggs of 140 hens collected for 30 days was studied using duplex PCR of certain fragments of sex chromosomes. Additional 894 dead embryos over a period of 21 days of incubation were also investigated to verify the sex ratio of the dead embryos. The sex of the early dead embryos was identified using this molecular sexing technique. The sex ratio of the hatch chicks and the total offspring of the hens investigated in this experiment did not differ from the expected sex ratio (i.e., 1:1)., However, the number of female dead embryos was significantly more than that of males. The data indicated that the different physiologic function of males and females contributed to female-biased mortality during incubation. It was also found by further analysis that the sex ratios of the offspring of some hens were significantly biased to female or male over the period investigated, which suggested that the sex ratio of offspring might be influenced by the maternal condition to some degrees. |
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| Keywords: | chicken offspring sex ratio sex identification |
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