| 枯草芽胞杆菌与氟环唑联用对禾谷镰孢霉的增效作用机制 |
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| 引用本文： | 毕秋艳,马志强,韩秀英,张小风,王文桥,赵建江.枯草芽胞杆菌与氟环唑联用对禾谷镰孢霉的增效作用机制[J].植物保护学报,2015,42(3):404-409. |
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| 作者姓名： | 毕秋艳 马志强 韩秀英 张小风 王文桥 赵建江 |
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| 作者单位： | 河北省农林科学院植物保护研究所,河北省农业有害生物综合防治工程技术研究中心,农业部华北北部作物有害生物综合治理重点实验室,保定071000 |
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| 基金项目： | 国家公益性行业(农业)科研专项(201303016) |
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| 摘 要： | 为验证枯草芽胞杆菌与氟环唑联用对禾谷镰孢霉的增效作用,用平板倒扣法和固体平板扩散法—打孔法测定菌药联用后枯草芽胞杆菌挥发性物质、抗真菌蛋白对禾谷镰孢霉的抑菌活性,观察抗真菌蛋白对禾谷镰孢霉菌丝形态的影响,并用刚果红染色法测定菌药联用后枯草芽胞杆菌纤维素酶活性。结果表明:菌药联用对禾谷镰孢霉的最高抑菌活性可达74.44%,表现增效和持效作用;枯草芽胞杆菌挥发性物质和抗真菌蛋白分别与氟环唑联用均可增强禾谷镰孢霉抑菌活性;菌药联用后枯草芽胞杆菌纤维素酶活性提高。研究表明枯草芽胞杆菌与氟环唑联用的增效机制是增强枯草芽胞杆菌挥发性物质对禾谷镰孢霉的抑菌活性,抗真菌蛋白活性的提高加强了禾谷镰孢霉菌丝溶解的程度,纤维素酶活性的增强提高了禾谷镰孢霉空间竞争作用和生物防治潜能。
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| 关 键 词： | 枯草芽胞杆菌 禾谷镰孢霉 氟环唑 联用 增效机制 |
| 收稿时间： | 2014/6/17 0:00:00 |
Preliminary exploration of the synergistic mechanism of Bacillus subtilis combined with epoxiconazole against Fusarium graminearum |
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| Bi Qiuyan,Ma Zhiqiang,Han Xiuying,Zhang Xiaofeng,Wang Wenqiao and Zhao Jianjiang.Preliminary exploration of the synergistic mechanism of Bacillus subtilis combined with epoxiconazole against Fusarium graminearum[J].Acta Phytophylacica Sinica,2015,42(3):404-409. |
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| Authors: | Bi Qiuyan Ma Zhiqiang Han Xiuying Zhang Xiaofeng Wang Wenqiao and Zhao Jianjiang |
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| Institution: | Key Laboratory of Integrated Pest Management on Crops in Northern Region of North China, Ministry of Agriculture; Integrated Pest Management Centre of Hebei Province; Institute of Plant Protection, Hebei Academy of Agricultural and Forestry Sciences, Baoding 071000, Hebei Province, China;Key Laboratory of Integrated Pest Management on Crops in Northern Region of North China, Ministry of Agriculture; Integrated Pest Management Centre of Hebei Province; Institute of Plant Protection, Hebei Academy of Agricultural and Forestry Sciences, Baoding 071000, Hebei Province, China;Key Laboratory of Integrated Pest Management on Crops in Northern Region of North China, Ministry of Agriculture; Integrated Pest Management Centre of Hebei Province; Institute of Plant Protection, Hebei Academy of Agricultural and Forestry Sciences, Baoding 071000, Hebei Province, China;Key Laboratory of Integrated Pest Management on Crops in Northern Region of North China, Ministry of Agriculture; Integrated Pest Management Centre of Hebei Province; Institute of Plant Protection, Hebei Academy of Agricultural and Forestry Sciences, Baoding 071000, Hebei Province, China;Key Laboratory of Integrated Pest Management on Crops in Northern Region of North China, Ministry of Agriculture; Integrated Pest Management Centre of Hebei Province; Institute of Plant Protection, Hebei Academy of Agricultural and Forestry Sciences, Baoding 071000, Hebei Province, China;Key Laboratory of Integrated Pest Management on Crops in Northern Region of North China, Ministry of Agriculture; Integrated Pest Management Centre of Hebei Province; Institute of Plant Protection, Hebei Academy of Agricultural and Forestry Sciences, Baoding 071000, Hebei Province, China |
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| Abstract: | In order to demonstrate the synergism of Bacillus subtilis combined with epoxiconazole against Fusarium graminearum, the antimicrobial activities of volatile substances and antifungal proteins of B. subtilis were determined with plate back-off method and solid plate diffusion method-punch method. The effects of antifungal proteins on mycelial morphology of F. graminearum were observed by microscopy. The activity of the cellulase of B. subtilis combined with epoxiconazole was also measured by Congo red staining method. The results showed that the highest antimicrobial activity of B. subtilis combined with epoxiconazole against F. graminearum was 74.44%, which suggested that the composite function was synergistic and persistent. The highly antimicrobial activities of volatile substances or the antifungal protein combined with epoxiconazole against F. graminearum was increased significantly. The activity of the cellulase of B. subtilis combined with epoxiconazole was also enhanced significantly. The synergistic mechanism of B. subtilis combined with epoxiconazole against F. graminearum was that the antimicrobial activity of the volatile substances was strengthened and the highest antimicrobial activities of the antifungal protein made the mycelia of F. graminearum discontinuously dissolve. At the same time, the activity of the cellulase of B. subtilis combined with epoxiconazole could enhance the competition for space and biotic potential against F. graminearum. |
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| Keywords: | Bacillus subtilis Fusarium graminearum epoxiconazole combination synergistic mechanism |
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