| PP_(333)、GA_3和BA对马铃薯试管苗生长调节作用的研究 |
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| 引用本文： | 李玉巧,朱鹿鸣.PP_(333)、GA_3和BA对马铃薯试管苗生长调节作用的研究[J].作物学报,1994,20(1):59-66. |
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| 作者姓名： | 李玉巧 朱鹿鸣 |
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| 作者单位： | 江苏省林业科学研究所植物微繁殖技术中心!江苏南京211153 |
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| 摘 要： | 针对马铃薯试管苗在继代培养中,苗生长细长、瘦弱和叶嫩黄等现象,在培养马铃薯的培养基中附加不同浓度的生长调节剂PP_(333)(mg/1,单位下同)、GA_3、BA、GA_3+BA、PP_(333)+GA_3、PP_(333)+BA和PP_(333)+GA_3+BA等进行培养壮苗的试验.加入PP_(333)各处理对试管苗的高、茎粗和增绿效应明显,但高浓度的PP_(333)对试管苗生长过于抑制;加GA_3对PP_(333)有拮抗作用.适宜浓度的PP_(333)+GA_3+BA能培养壮苗,并促进侧芽分化,增加叶面积、叶绿素含量和干物质的累积.各处理对马铃薯试管苗的抑制生长效应为PP_(333)>PP_(333)+BA>PP_(333)+GA_3>PP_(333)+GA_3+BA>BA>GA_3>GA_3+BA;生理效应和移栽苗成活率的顺序为PP_(333)+GA_3+BA>PP_(333)+GA_3>GA_3+BA>PP_(333)+BA>GA_3>BA>PP_(333).
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| 关 键 词： | 马铃薯试管苗 植物生长调节剂 PP_（333） GA_3 BA矮壮 |
| 收稿时间： | 1991-10-31 |
| 修稿时间： | 1992-12-20 |
Study on the Effects of Growth Regulator PP_(333),GA_3 and BA on Potato Seedlings Cultured in vitro |
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| Li Yu-qiao Zhu Lu-ming.Study on the Effects of Growth Regulator PP_(333),GA_3 and BA on Potato Seedlings Cultured in vitro[J].Acta Agronomica Sinica,1994,20(1):59-66. |
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| Authors: | Li Yu-qiao Zhu Lu-ming |
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| Institution: | The Centre of Plant Micropropagation of Jiangsu Province, Forestry Research Institute of Jiangsu Province Nanjing,211153 |
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| Abstract: | In order to reduce the risk of leggy and feeble and leaf yollowish and increase the survival rate of seedlings of Solanum tuberosum during the transptanting of the plantlets, an experiment was carried out to study the effects of chemical control of Paclobutrazol (PP333), Gibberellin (GA3) and promalin (BA) on the cultured potato plantlets in vitro by adding PP333 1. 0mg/l (unit (mg/l) ,the same as below) +GA3 0. 7+BA 0. 2,PP3331. 0+GA3 0. 7, PP3331. 0+BA 0. 2,GA3 0. 7+BA0. 2,GA30. 7,PP3331. 0 and BA 0. 2 in the basic medium(MS). According to the experi-mental results,the decrease of growth rate in potato seedlings was the character of significant growing-control effects of PP333. This test initiated to determine the deleterious effects following high concentration of PP333which could be overcome by GA3. By observations,PP333 and exogenous GA3 had the antagonism obviously on the growth of potato seedling in vitro. The regulators at suitable concentration of PP3331. 0+GA30. 7 + BA0. 2 could inhibit the growth of seedling and promoted the stem diameter, lateral bud number, leaf area chlorophyll content, and biomass of plantlets dry weight of the seedlings in vitro.The test results also showed: 1. the growth-controlling effect on potato seedling in vitro with 7 treatments during seedling growth in 40 days were PP333>PP333+BA>PP333+GA3> PP333+GA3+BA>BA>GA3>GA3+BA. 2. the physiological effect on growth of seedling quality (included chlorophyll content and biomass of seedling dry weight) and the survival rate of potato seedling during transplanting of the plantlets in vitro with 7 treatments were PP333+GA3 +BA>PP333+GA3>GA3+BA>PP333+BA>GA3>BA>PP333. |
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| Keywords: | Solanum tuberosum seedling in vitro Plant regulator PP333 GA3 BA Dwarfism |
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