| 中华绒螯蟹Myostatin基因在蜕皮过程中的表达分析 |
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| 引用本文： | 王伟,吴旭干,徐蕾,姚琴琴,成永旭.中华绒螯蟹Myostatin基因在蜕皮过程中的表达分析[J].上海海洋大学学报,2015,24(5):662-667. |
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| 作者姓名： | 王伟 吴旭干 徐蕾 姚琴琴 成永旭 |
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| 作者单位： | 中国水产科学研究院，东海水产研究所,上海海洋大学,上海海洋大学,上海海洋大学,上海海洋大学 |
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| 基金项目： | 上海市科委科研计划项目 (13231203504,13320502100)；科技部科技型中小企业技术创新项目(14C26213201214)；国家农业成果转化项目(2012GB2C000147)；上海高校水产学一流学科建设项目(沪教科2012-62) |
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| 摘 要： | 本研究通过荧光定量PCR（qRT-PCR）技术对中华绒螯蟹Myostatin（EsMSTN）基因在不同组织及蜕皮阶段的表达情况进行了分析。结果显示，(1) EsMSTN在C期中华绒螯蟹肌肉组织中的基因表达水平最高，胸神经节和脑神经节中也有较高水平的表达，暗示EsMSTN基因的功能并不局限于肌肉生长的调节。(2) 在不同蜕皮阶段，肌肉中EsMSTN的基因表达水平在AB期最高，D期最低，推测EsMSTN可能正向调节中华绒螯蟹肌肉生长，参与肌肉组织蜕皮后的生长以及蜕皮前的萎缩过程；肝胰腺、上皮组织中EsMSTN的最高基因表达水平都出现蜕皮后的AB期，C期或D期相对表达量最低，这可能与上述器官的细胞增殖主要发生在AB期有关；胸神经节中EsMSTN的表达水平在E期最高，D期最低，暗示EsMSTN正向调节胸神经节中的神经发生。
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| 关 键 词： | 中华绒螯蟹 Myostatin（MSTN） 表达分析 蜕皮 |
| 收稿时间： | 2014/12/29 0:00:00 |
| 修稿时间： | 6/4/2015 12:00:00 AM |
Expression analysis of Myostatin during molting cycle in Eriocheir sinensis |
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| WANG Wei,WU Xugan,XU Lei,YAO Qinqin and CHENG Yongxu.Expression analysis of Myostatin during molting cycle in Eriocheir sinensis[J].Journal of Shanghai Ocean University,2015,24(5):662-667. |
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| Authors: | WANG Wei WU Xugan XU Lei YAO Qinqin and CHENG Yongxu |
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| Institution: | East China Sea Fisheries Research Institute, Chinese Academyof Fishery Sciences,Shanghai Ocean University,Shanghai Ocean University,Shanghai Ocean University,Shanghai Ocean University |
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| Abstract: | In present study, the expression differences of myostatin (MSTN) in various tissues of Eriocheir sinensis were analyzed by qRT-PCR during molting cycle. The results showed that, (1) EsMSTN (Eriocheir sinensis MSTN) had its highest expression level in muscle, while higher expression levels were also observed in thoracic ganglia and brain ganglia at stage C, suggesting its physiological function was not be restricted to muscle. (2) EsMSTN was expressed highest at AB and lowest at D stage of molting cycle in muscle, suggesting that EsMSTN might be a positive growth regulator in E. sinensis, which involved in the significant expansion and severe atrophy of the muscle at AB stage and D stage, respectively. Whereas in hepatopancreas and epithelium, EsMSTN mRNA expression levels were highest at AB stage, and lowest at C or D stage, due probably to cell proliferation at AB stage in these tissues. In addition, the peak value of EsMSTN mRNA in thoracic ganglia occurred at stage AB and its lowest expression level was observed at stage D, which implied that EsMSTN may regulate the nerogenesis positively in this tissue. |
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| Keywords: | Eriocheir sinensis Myostatin (MSTN) expression analysis molting |
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