| 薄荷离体培养及植株再生的研究 |
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| 引用本文： | 钱森和,厉荣玉,王洲,郭恭睿,杨超英.薄荷离体培养及植株再生的研究[J].作物杂志,2008(6). |
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| 作者姓名： | 钱森和 厉荣玉 王洲 郭恭睿 杨超英 |
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| 作者单位： | 1. 安徽工程科技学院生化系,241000,安徽芜湖
2. 皖南医学院 |
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| 基金项目： | 安徽工程科技学院本科生科研资助项目 |
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| 摘 要： | 以薄荷茎段为外植体,研究了不同植物激素对薄荷愈伤组织的诱导及其增殖、不定芽分化和诱导生根的影响。结果表明,愈伤诱导最适培养基为MS 6-BA1.5mg/L 2,4-D0.5mg/L NAA0.5mg/L,其愈伤组织生长良好;最佳愈伤增殖培养基为MS NAA1.0mg/L或MS IAA2.0mg/L,其褐化率均较低;最佳诱导分化培养基为MS 6-BA2.0mg/L NAA0.1mg/L,分化率达77%;生根培养基为1/2MS NAA2.0mg/L,且根较为粗壮。
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| 关 键 词： | 薄荷 离体培养 诱导 植株再生 |
Tissue Culture and Plantlet Regeneration of Mint |
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| Qian Senhe,Li Rongyu,Wang Zhou,Guo Gongrui,Yang Chaoying.Tissue Culture and Plantlet Regeneration of Mint[J].Crops,2008(6). |
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| Authors: | Qian Senhe Li Rongyu Wang Zhou Guo Gongrui Yang Chaoying |
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| Abstract: | The stem segments of Mentha canadensis were used as explants to study the effect of plant regulators on callus induction,callus multiplication,adventitious buds differentiation and rootage.The results showed that MS 1.5mg/L 6-BA 0.5mg/L 2,4-D 0.5mg/L NAA was the most appropriate media for callus induction for callus growth.MS 1.00mg/L NAA or MS 2.00mg/L IAA was the most appropriate media for callus multiplication with low browning rate.MS 2.0mg/L 6-BA 0.1mg/L NAA was the most appropriate media for differentiation of adventitious buds,with the efficiency of 77%.1/2MS 2.0mg/L NAA was the most appropriate media for rooting. |
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| Keywords: | Mentha canadensis In vitro culture Induction Plantlet regenerateion |
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