|
|||||
|
|
| 香蕉茉莉酸合成关键酶基因MaOPR 的克隆和表达分析 | |
| 引用本文： | 许奕,徐碧玉,宋顺,刘菊华,张建斌,贾彩红,金志强.香蕉茉莉酸合成关键酶基因MaOPR 的克隆和表达分析[J].园艺学报,2013,40(2):237-246. |
| 作者姓名： | 许奕 徐碧玉 宋顺 刘菊华 张建斌 贾彩红 金志强 |
| 作者单位： | （1 中国热带农业科学院海口实验站，海口 570102；2 海南省香蕉遗传改良重点实验室，海口 570102；3 中国热带农 业科学院热带生物技术研究所，农业部热带作物生物学与遗传资源利用重点实验室，海口 571101） |
| 基金项目： | 现代农业产业技术体系建设专项资金项目(CARS-32);中央级公益性科研院所基本科研业务费专项(ITBB110202);中央级公益性科研院所基本科研业务费专项(ITBB110101);‘十二五’农村领域国家科技计划课题(2011AA10020605) |
| 摘 要： | 从‘巴西’香蕉（Musa acuminata L. AAA group‘Brazilian’）根的cDNA 文库中获得了一段
12–氧–植物二烯酸还原酶OPR（12-oxo-phytodienoic acid reductase）基因的片段，RACE 扩增获得全长，
命名为MaOPR。该基因全长1 512 bp，存在一个完整的开放阅读框1 287 bp，编码429 个氨基酸。生物
信息学分析表明，该蛋白属稳定蛋白，等电点为8.11，具有一个活性位点，一个基质结合位点，一个黄素
单核苷酸结合位点。序列预测分析该蛋白亚细胞定位为细胞质，其不属于跨膜蛋白且不存在信号肽。通
过和已知植物的12–氧–植物二烯酸还原酶基因相比，同源性达到66%以上。其中与苜蓿、谷子、粳稻、
紫云英的OPR 编码的氨基酸序列的同源性均为71%。器官特异性分析表明，MaOPR 在香蕉的根、茎、
叶片、花和果实中均有所表达，其中在茎和果实中表达量较高。通过对其在ABA 抑制剂、乙烯、枯萎病
胁迫下的表达结果分析显示，该基因响应以上3 种胁迫。 |
| 关 键 词： | 香蕉 12–氧–植物二烯酸还原酶基因 序列分析 荧光定量PCR |
Molecular Cloning and Expression Analysis of MaOPR Gene from Banana |
|
| XU Yi,XU Bi-yu,SONG Shun,LIU Ju-hua,ZHANG Jian-bin,JIA Cai-hong,and JIN Zhi-qiang.Molecular Cloning and Expression Analysis of MaOPR Gene from Banana[J].Acta Horticulturae Sinica,2013,40(2):237-246. | |
| Authors: | XU Yi XU Bi-yu SONG Shun LIU Ju-hua ZHANG Jian-bin JIA Cai-hong and JIN Zhi-qiang |
| Institution: | （1Haikou Experimental Station，Chinese Academy of Tropical Agricultural Sciences，Haikou 570102，China；2Hainan Key Laboratory of Banana Genetic Improvement，Haikou 570102，China；3Key Laboratory of Biology and Genetic Resources of Tropical Crops，Ministry of Agriculture；Institute of Tropical Bioscience and Biotechnology，Chinese Academy of Tropical Agricultural Sciences，Haikou 571101，China） |
| Abstract: | Through RACE approaches and bioinformatics analysis，the full-length cDNA of the cysteine synthase gene，named as MaOPR（12-oxo-phytodienoic acid reductase），was obtained from root of banana（Musa acuminata L. AAA group‘Brazilian’）cDNA library，and its sequence characters were also analyzed. The full length of this gene was 1 512 bp，it had an 1 287 bp open reading frame in length encoding 429 amino acid. The result of bioinformatics showed that this protein was a stable protein with the active sites，the matrix attachment sites and the flavin mononucleotide binding sites，which pI is 8.11. The sequence prediction showed that it located in the cytoplasm，it was not a transmembrane protein and had no signal peptide. Compared with other known plants，the homology of MaOPR was more than 66%. Amino acids homology analysis indicated that MaOPR had 71% similarity compared with Medicago sativa，Setaria italic，Oryza sativa，Astragalus sinicus. RT-PCR analysis showed that MaOPR was constitutively expressed in roots，stems，leaves，flowers and fruits. The expression level was the highest in fruits. Analysis showed that MaOPR responsed to the stress including the ABA inhibitor，ethylene and wilt disease stress. |
| Keywords: | banana 12-oxo-phytodienoic acid reductase sequence character real-time PCR |
| 本文献已被 CNKI 等数据库收录！ | |
| 点击此处可从《园艺学报》浏览原始摘要信息 | |
| 点击此处可从《园艺学报》下载免费的PDF全文 | |