| 黄瓜果皮光泽性状的遗传分析及基因定位研究 |
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| 引用本文： | 董邵云,苗晗,张圣平,王烨,王敏,刘书林,顾兴芳.黄瓜果皮光泽性状的遗传分析及基因定位研究[J].园艺学报,2013,40(2):247-254. |
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| 作者姓名： | 董邵云 苗晗 张圣平 王烨 王敏 刘书林 顾兴芳 |
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| 作者单位： | 中国农业科学院蔬菜花卉研究所，北京 100081 |
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| 基金项目： | 国家‘973’计划项目(2012CB113900);国家‘863’计划课题(2012AA100105);现代农业产业技术体系建设专项资金项目(CARS-25);农业部园艺作物生物学与种质创制重点实验室项目 |
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| 摘 要： | 以黄瓜（Cucumis sativus L.）果皮有光泽自交系‘1101’（P1）为母本，以3 个无光泽自交
系‘1116’（P2）、‘9930’（P3）、‘1107’（P4）为父本，分别构建6 世代遗传群体，对黄瓜果皮光泽性状
进行遗传规律分析和基因定位研究。结果表明，黄瓜果皮有光泽性状由显性单基因G 控制，有光泽对无
光泽为显性。利用‘1101’ב1116’的F2 群体，结合分离群体分组分析法（bulked segregate analysis，
BSA）筛选得到了30 对与黄瓜果皮有光泽基因G 相关的SSR 标记，将该基因定位到黄瓜第5 染色体上，
侧翼标记为CS28 和SSR15818，遗传距离分别为2.0 cM 和6.4 cM。两侧翼标记之间的物理距离为454 kb，
在该区域中共预测了177 个候选基因。
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| 关 键 词： | 黄瓜 果皮光泽 遗传分析 SSR 基因定位 |
Genetic Analysis and Gene Mapping of Glossy Fruit Skin in Cucumber |
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| DONG Shao-yun,MIAO Han,ZHANG Sheng-ping,WANG Ye,WANG Min,LIU Shu-lin,and GU Xing-fang.Genetic Analysis and Gene Mapping of Glossy Fruit Skin in Cucumber[J].Acta Horticulturae Sinica,2013,40(2):247-254. |
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| Authors: | DONG Shao-yun MIAO Han ZHANG Sheng-ping WANG Ye WANG Min LIU Shu-lin and GU Xing-fang |
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| Institution: | Institute of Vegetable and Flowers，Chinese Academy of Agricultural Sciences，Beijing 100081，China |
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| Abstract: | Glossy fruit skin is one of the highly valuable appearance quality traits related to market
values of cucumber（Cucumis sativus L.）. Inbred line‘1101’（glossy fruit skin）and inbred line‘1116’
（dull fruit skin），‘9930’（dull fruit skin），‘1107’（dull fruit skin）were used as experiment materials
for genetic analysis and gene mapping in this study. Genetic analysis showed that a single dominant
nuclear gene，G（glossy fruit skin），determines the glossy fruit skin trait in cucumber. Bulked segregate
analysis（BSA）and simple sequence repeat （SSR）technologies were employed to map G gene of cucumber
in‘1101’ב1116’F2 population. G gene was mapped to a linkage group with 30 SSR markers，
corresponding to chromosome 5 of cucumber. The flanking markers CS28 and SSR15818 were linked to
the G gene with genetic distances of 2.0 and 6.4 cM，respectively. The physical distance between the two
markers was 454 kb based on the whole genome sequence of cucumber，and there are 177 candidate genes
in this region. |
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| Keywords: | cucumber glossy fruit skin genetic analysis SSR marker gene mapping |
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