| ApNPV侵染对柞蚕蛹体内保护酶活性的影响 |
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| 引用本文： | 石生林,张涛,李群,王学英.ApNPV侵染对柞蚕蛹体内保护酶活性的影响[J].沈阳农业大学学报,2008,39(5). |
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| 作者姓名： | 石生林 张涛 李群 王学英 |
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| 作者单位： | 沈阳农业大学,生物科学技术学院,沈阳,110161 |
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| 基金项目： | 沈阳农业大学中青年硕士导师基金项目(2006) |
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| 摘 要： | 为研究柞蚕核型多角体病毒（Antheraec pernyi nucleopolyhedrovirus，ApNPV）侵染后病毒与宿主间的拮抗关系，测定ApNPV侵染后柞蚕蛹体内SOD、CAT和PO活性变化。结果表明：ApNPV侵染使柞蚕蛹体内SOD活性呈先下降再上升后又下降的变化趋势，接种后48h和96h是酶活性变化的两个转折点，48h是酶活性差值低谷，雌、雄平均较对照低622U·mL^-1；96h是酶活性差值高峰．雌、雄平均较对照高850．5U·mL^-1。CAT活性变化呈先上升后下降的趋势，接种后48h是酶活性变化的转折点，雌、雄平均较对照高309．5U·mL^-1；72h后酶活性变化趋于平稳。PO活性亦呈先上升后下降的变化趋势，接种后48h是酶活性变化转折点，雌、雄平均较对照高2173．5U·mL^-1。雌、雄蛹间酶系活性变化趋势相似但程度不同，雌蛹酶活性变化幅度大于雄蛹。
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| 关 键 词： | 柞蚕 核型多角体病毒 侵染 酶活性 |
Effects of ApNPV Infection on Superoxide Dismutase,Catalase and Phenoloxidase of Antheraea pernyi Pupa |
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| SHI Sheng-lin,ZHANG Tao,LI Qun,WANG Xue-ying.Effects of ApNPV Infection on Superoxide Dismutase,Catalase and Phenoloxidase of Antheraea pernyi Pupa[J].Journal of Shenyang Agricultural University,2008,39(5). |
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| Authors: | SHI Sheng-lin ZHANG Tao LI Qun WANG Xue-ying |
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| Abstract: | To study the antagonistic relationship between Antheraea pernyi nucleopolyhedrovirus(ApNPV)and its host under the condition of ApNPV infection,the activities of superoxide dismutase(SOD),catalase(CAT) and phenoloxidase(PO) of ApNPV infected Antheraea pernyi pupa were determined.The results showed that due to ApNPV infection,the SOD activities in pupa decreased at first and then increased and finally decreased again.The two turning points of SOD activity lay at hours 48 and 96 after injection.At 48 h,the mea... |
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| Keywords: | Antheraea pernyi nucleopolyhedrovirus infection enzymic activity |
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