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| 陆地棉矮化突变体Ari1327的矮化机理研究 | |
| 引用本文： | 王新坤,孙君灵,潘兆娥,张超,何守朴,贾银华,周忠丽,唐灿明,杜雄明.陆地棉矮化突变体Ari1327的矮化机理研究[J].棉花学报,2012,24(4):293-302. |
| 作者姓名： | 王新坤 孙君灵 潘兆娥 张超 何守朴 贾银华 周忠丽 唐灿明 杜雄明 |
| 作者单位： | 1. 中国农业科学院棉花研究所/农业部棉花遗传改良重点实验室,河南 安阳 455000;南京农业大学,江苏 南京 210095 2. 中国农业科学院棉花研究所/农业部棉花遗传改良重点实验室,河南 安阳,455000 3. 四川省农业科学院,四川 简阳,641400 4. 南京农业大学,江苏 南京,210095 |
| 基金项目： | 国家重点基础研究发展计划(973计划)项目 |
| 摘 要： | Ari1327是从美国引进的陆地棉种质Ari971经60Coγ射线照射后得到的一个新型矮化突变体。Ari1327在萌发期和子叶期就表现出矮化性状。在现蕾期，突变体的株高、节间数和节间平均长度均小于野生型，差异达到极显著水平，节间平均长度的缩短导致了突变体株高的降低。突变体主茎节间纵向细胞的长度要显著小于野生型，横向细胞的直径与野生型差异不显著，节间细胞伸长受抑制导致了突变体节间平均长度的缩短。突变体中IAA、GA3和ABA 含量高于野生型，外施GA3能使其株高恢复至野生型水平，外施BR和IAA不能使其株高恢复到正常水平，较低浓度的IAA对突变体的株高和节间长度就开始产生抑制作用。突变可能导致了IAA合成相关基因的过量表达，突变体内源IAA含量急剧升高而抑制了植株生长，使株高降低。 |
| 关 键 词： | 棉花（G.hirsutum L.） 矮化 机理 |
| 收稿时间： | 2011-05-04; |
Study on the Dwarf Mechanism of an Upland Cotton (G.hirsutum L.) Dwarf Mutant,Ari1327 |
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| WANG Xin-kun , SUN Jun-ling , PAN Zhao-e , ZHANG Chao , HE Shou-pu , JIA Yin-hua , ZHOU Zhong-li , TANG Can-ming , DU Xiong-ming.Study on the Dwarf Mechanism of an Upland Cotton (G.hirsutum L.) Dwarf Mutant,Ari1327[J].Cotton Science,2012,24(4):293-302. | |
| Authors: | WANG Xin-kun SUN Jun-ling PAN Zhao-e ZHANG Chao HE Shou-pu JIA Yin-hua ZHOU Zhong-li TANG Can-ming DU Xiong-ming |
| Institution: | 1(1.Cotton Research Institute,CAAS / Key Laboratory of Cotton Genetics Improvement,Ministry of Agriculture,Anyang,Henan 455000,China;2.Nanjing Agricultural University,Nanjing,Jiangsu 210095,China;3.Sichuan Academy of Agricultural Sciences,Jianyang,Sichuan 641400,China) |
| Abstract: | Plant height is an important agronomic trait in upland cotton.Ari1327 is a dwarf mutant derived from an American upland cotton variety,Ari971,by 60Co γ-ray irradiation.Ari1327 exhibits the dwarf trait during the germination and cotyledon periods.During the budding period,the plant height,internode number and internode length of Ari1327 were significantly(P < 0.01) smaller or fewer than in the wild type.Therefore,the mutant dwarfism was due to the decrease of average internode length.The longitudinal cell length of mutant internodes was significantly shorter than in the wild type(P < 0.01).The difference in transverse cell diameter between the mutant and the wild type was not significant.Thus,the shorter internodes were induced by inhibition of internode cells.The IAA,GA3 and ABA content of Ari1327 was higher than in the wild type.The height of Ari1327 plants could be restored to wild type levels by exogenous GA3 application.Exogenous BR and IAA had no effect on Ari1327.Due to over-expression of genes in the IAA synthetic pathway,the IAA content in Ari1327 was remarkably increased.High-density IAA inhibits plant growth;thus,the plant exhibits a dwarf phenotype. |
| Keywords: | cotton(G hirsutum L ) dwarfism mechanism |
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