| 2-羟基-4-甲氧基苯甲醛和2-羟基-4-甲氧基苯甲酸对菜粉蝶多酚氧化酶的抑制作用 |
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| 引用本文： | 王勤,柯莉娜,薛超彬,贺量,罗万春,陈清西.2-羟基-4-甲氧基苯甲醛和2-羟基-4-甲氧基苯甲酸对菜粉蝶多酚氧化酶的抑制作用[J].广西农业生物科学,2006,25(2):155-159. |
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| 作者姓名： | 王勤 柯莉娜 薛超彬 贺量 罗万春 陈清西 |
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| 作者单位： | 1. 厦门大学,生命科学学院,福建,厦门,361005
2. 山东农业大学,农药毒理与应用技术省级重点实验室,山东,泰安,271018 |
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| 基金项目： | 福建省重点科技项目(2004N002)，国家自然科学基金项目(30570408) |
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| 摘 要： | 测定了2羟-基-4-甲氧基苯甲醛和2羟-基-4-甲氧基苯甲酸两种效应物对菜粉蝶多酚氧化酶(po lypheno lox idase,简称PPO,EC.1.14.18.1)催化L-多巴(L-DOPA)氧化活力的抑制作用。结果表明,这两种效应物对该酶的活性均有明显的抑制作用,抑制中浓度(IC50)分别为2.71和5.66 mm o l/L,抑制常数分别为2.82和3.35 mm o l/L;2羟-基-4-甲氧基苯甲醛对该酶的抑制作用显著高于2羟-基-4-甲氧基苯甲酸的;这两种抑制剂对酶的抑制作用机理完全不同,2羟-基-4-甲氧基苯甲醛对酶的作用表现为混合型抑制,而2羟-基-4-甲氧基苯甲酸对酶的作用表现为竞争性抑制。研究结果为设计以该酶为靶标的杀虫剂提供理论依据。
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| 关 键 词： | 菜粉蝶 多酚氧化酶 2羟-基-4-甲氧基苯甲醛 2羟-基-4-甲氧基苯甲酸 抑制作用 |
| 文章编号： | 1008-3464(2006)02-0155-05 |
| 修稿时间： | 2005年6月10日 |
Inhibitory effects of 2-hydroxy-4-methoxy-benzaldehyde and 2-hydroxy-4-methoxy-benzoic acid on polyphenoloxidase of Pieris rapae (L.) |
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| WANG Qin,KE Li-na,XUE Chao-bin,HE Liang,LUO Wan-chun,CHEN Qing-xi.Inhibitory effects of 2-hydroxy-4-methoxy-benzaldehyde and 2-hydroxy-4-methoxy-benzoic acid on polyphenoloxidase of Pieris rapae (L.)[J].Journal of Guangxi Agricultural and Biological Science,2006,25(2):155-159. |
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| Authors: | WANG Qin KE Li-na XUE Chao-bin HE Liang LUO Wan-chun CHEN Qing-xi |
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| Abstract: | Inhibitory effects of 2-hydroxy-4-methoxy-benzaldehyde and 2-hydroxy-4-methoxy-benzoic acid on polyphenoloxidase(PPO) from larvae of Pieris rapae(L.) were studied.The results showed that both compounds could obviously inhibit enzyme activity for oxidation of L-DOPA,and their IC_(50)(inhibitor concentration leading to 50% activity lost) were 2.71 and 5.66 mmol/L,respectively.Their inhibition constants were 2.82 and 3.35 mmol/L.Inhibitory ability of 2-hydroxy-4-methoxy-benzaldehyde was much higher than that of 2-hydroxy-4-methoxy-benzoic acid.Inhibitory mechanisms of the two compounds were very different.The inhibition of 2-hydroxy-4-methoxy-benzaldehyde was mixed type while that of 2-hydroxy-4-methoxy-benzoic acid was competitive type. |
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| Keywords: | Pieris rapae(L ) polyphenoloxidase 2-hydroxy-4-methoxy-benzaldehyde 2-hydroxy-4-methoxy-benzoic acid inhibition |
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