| 路易斯安娜鸢尾组培和苗期生长规律初步研究 |
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| 引用本文： | 朱旭东,田松青,周玉珍,娄晓鸣,韦庆华.路易斯安娜鸢尾组培和苗期生长规律初步研究[J].福建林业科技,2009,36(3). |
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| 作者姓名： | 朱旭东 田松青 周玉珍 娄晓鸣 韦庆华 |
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| 作者单位： | 苏州农业职业技术学院,江苏苏州,215008 |
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| 基金项目： | 江苏省"青蓝工程"资助项目，苏州市科技创新项目 |
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| 摘 要： | 以路易斯安娜鸢尾顶芽为外植体,利用不同激素配比诱导不定芽,定期测量组培苗株高、蓬径、主茎长和叶片数,观察组培苗的染色体数、叶片结构和开花性状等。结果表明:路易斯安娜鸢尾顶芽诱导率最高的培养基为MS+6-BA 2.0 mg.L-1+NAA 0.5 mg.L-1,产生有效生产苗最多的培养基为MS+6-BA 0.5 mg.L-1+NAA 0.2 mg.L-1,最佳生根培养基为1/2MS+NAA 0.5 mg.L-1+活性碳0.5 g.L-1。2年生组培苗平均株高达到71.6 cm,平均蓬径达到60.9 cm,叶片维持在6~12片,冬季仍有绿叶。组培苗染色体个数、叶片通气组织、开花性状等均与母株一致,表明路易斯安娜鸢尾组培苗可保持其母株的观赏性状和水生习性,可通过组培快速繁殖大规模生产优质种苗。
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| 关 键 词： | 路易斯安娜鸢尾 组织培养 苗期生长 遗传性状 |
Elementary Study on Tissue Culture of Louisiana iris and Its Growth Law in Seedling Period |
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| ZHU Xu-dong,TIAN Song-qing,ZHOU Yu-zhen,LOU Xiao-ming,WEI Qing-hua.Elementary Study on Tissue Culture of Louisiana iris and Its Growth Law in Seedling Period[J].Journal of Fujian Forestry Science and Technology,2009,36(3). |
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| Authors: | ZHU Xu-dong TIAN Song-qing ZHOU Yu-zhen LOU Xiao-ming WEI Qing-hua |
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| Institution: | ZHU Xu-dong,TIAN Song-qing,ZHOU Yu-zhen,LOU Xiao-ming,WEI Qing-hua(Suzhou Vocational Technology College of Agriculture,Suzhou 215008,Jiangsu,China) |
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| Abstract: | With top buds of Louisiana iris as explants,after inducing adventitious bud with different hormone compound,this paper measured the plant height,diameter of its leaves crown,stem length and leaf number of tissue culture seedling regularly.It observed chromosome number,leaf structure and blossom of tissue culture seedling.The results showed medium with highest top bud induction rate was MS+6-BA 2.0 mg·L-1+NAA 0.5 mg·L-1,the medium producing most effective productive seedling was MS+6-BA 0.5 mg·L-1+NAA 0.2 mg·L-1 and the best medium of rooting culture was 1/2MS+NAA0.5 mg·L-1+activated carbon 0.5 g·L-1.Average height of tissue culture seedlings reached 71.6 cm at the age of 2,average diameter of its leaves crown was 60.0 cm and leaves number was 6 to 12.It owned green leaves in winter.Chromosome number of tissue culture seedlings,leaves aerenchyma,flowering character were accordant with mother plant.L.iris tissue culture seedling could keep its mother plant's ornamental characters and hydrophytic behaviors.The fine quality of seedling could be produced in a large scale by tissue culture rapid propagation. |
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| Keywords: | Louisiana iris tissue culture growth in seedling period heredity character |
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