Isolation and restriction endonuclease analysis of a tetracycline resistance plasmid from Staphylococcus hyicus |
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| Authors: | S Schwarz H Blobel |
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| Institution: | Institut für Bakteriologie und Immunologie der Justus-Liebig-Universit?t Giessen, F.R.G. |
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| Abstract: | A plasmid of 4.550 kb, conferring resistance to tetracycline, was demonstrated in Staphylococcus hyicus cultures from piglets with exudative epidermidis. The plasmid-encoded properties were determined both by curing and interspecific protoplast transformation experiments. The tetracycline resistance (TET) plasmid, designated pST1, was characterized by restriction endonuclease analysis and a preliminary restriction map was constructed. The pST1 plasmid was demonstrated in 19 (57.6%) of 33 S. hyicus cultures by Southern blot hybridization. It was also detectable by electron microscopy. |
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