| 最佳组合溶磷细菌的筛选及培养条件的优化 |
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| 引用本文： | 乔志伟,洪坚平,刘超.最佳组合溶磷细菌的筛选及培养条件的优化[J].中国土壤与肥料,2017(5):141-146. |
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| 作者姓名： | 乔志伟 洪坚平 刘超 |
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| 作者单位： | 1. 安顺学院资源环境与工程学院,贵州 安顺,561000;2. 山西农业大学资源环境学院,山西 太谷,030801 |
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| 基金项目： | 国家自然科学基金项目(31272257)，贵州省科技厅、安顺市人民政府、 安顺学院三方联合基金黔科合LH字(2016 -7280号) |
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| 摘 要： | 试验研究了不同溶磷细菌的组合效应,筛选出最佳组合,并通过单因子试验和正交试验对最佳组合菌株的培养条件进行了优化。结果表明:溶磷细菌最佳组合为拉恩式菌(W2)+假单胞菌1(W3)+假单胞菌2(W4);W2+W3+W4以葡萄糖为碳源、硫酸铵为氮源培养液有效磷含量最大,为609.1 mg/L,比空白增加了18.4倍;正交试验表明W2+W3+W4的最适培养条件为葡萄糖15 g/L,硫酸铵为0.67 g/L,培养液初始p H值7,接菌量1%,经过进一步实验,W2+W3+W4在优化后培养条件下对磷酸三钙的溶解能力为664.29 mg/L,比在普通发酵培养液中的溶磷量显著增加55.19 mg/L(P0.05),为增强溶磷微生物在土壤中的竞争能力和溶磷能力提供理论基础。
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| 关 键 词： | 溶磷细菌 组合效应 最佳组合 正交试验 |
| 收稿时间： | 2016/12/4 0:00:00 |
| 修稿时间： | 2016/12/23 0:00:00 |
Screening the best combination of phosphobacteria and optimization of its culture conditions |
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| QIAO Zhi-wei,HONG Jian-ping,LIU Chao.Screening the best combination of phosphobacteria and optimization of its culture conditions[J].Soil and Fertilizer Sciences in China,2017(5):141-146. |
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| Authors: | QIAO Zhi-wei HONG Jian-ping LIU Chao |
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| Abstract: | The tricalcium phosphate-solubilizing ability of phosphobacteria and their combinations was tested using laboratory shake-flask culture. The culture broth of W2+W3+W4 produced the highest available P content, which was 609. 1 mg/L. Thus, W2+W3+W4 was selected as the optimal combination of phosphobacteria. Then the best combination strain culture conditions were optimized through the single factor test and orthogonal test. The results showed that W2+W3 +W4 had grea-test culture effective phosphorus content with glucose as carbon source and ammonium sulfate as nitrogen source, the content was 609. 1 mg/L and an increase of 18. 4 times than the control. Orthogonal test showed that W2+W3+W4 the optimal cul-ture conditions was 15 g/L glucose, 0. 67 g/L ammonium sulfate, culture medium initial pH 7, and 1% the inoculation quan-tity. After further experiments, the dissolve tricalcium phosphate content of W2+W3+W4 under the optimized was 664. 29 mg/L, which was 55. 19 mg/L (P< 0. 05) more than that of normal fermentation broth. |
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| Keywords: | soluble phosphorus bacteria combination effect the best combination orthogonal test |
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