| 秋水仙碱应用于小豆诱导变异初探 |
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| 引用本文： | 濮绍京,李金玉,张晶晶,赵波,金文林.秋水仙碱应用于小豆诱导变异初探[J].北京农学院学报,2005,20(4):5-8. |
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| 作者姓名： | 濮绍京 李金玉 张晶晶 赵波 金文林 |
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| 作者单位： | 北京农学院作物遗传育种研究所、农业应用新技术北京市重点实验室,北京,102206;北京农学院作物遗传育种研究所、农业应用新技术北京市重点实验室,北京,102206;北京农学院作物遗传育种研究所、农业应用新技术北京市重点实验室,北京,102206;北京农学院作物遗传育种研究所、农业应用新技术北京市重点实验室,北京,102206;北京农学院作物遗传育种研究所、农业应用新技术北京市重点实验室,北京,102206 |
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| 摘 要： | 将秋水仙碱浓度为0.2000%、0.1000%、0.0500%、0.0250%、0.0125%的水溶液分别处理萌动的京农5号小豆种子8、16、24 h,通过对M1世代植株成活率、株高降低比例、M2世代变幅及变异系数、诱导变异遗传力、M2世代出现的明显变异类型进行分析,探讨秋水仙碱诱变小豆的适宜浓度及处理时间。结果表明:(1)根据M1世代相对成株率,较适宜的诱变处理为0.2000%浓度8 h和0.1000%浓度24 h;(2)处理浓度对M1世代出苗和成株率的直接影响明显大于处理时间;(3)以50%相对成株率为标准时,8、16、24 h浸泡,所适宜的处理浓度分别为0.215%、0.156%、0.115%;(4)如以株高降低50%~60%为标准,则适宜浓度为0.0500%~0.2000%;(5)M2世代中,出现花粒和感白粉病等多种变异类型。
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| 关 键 词： | 小豆 秋水仙碱 诱变 |
| 文章编号： | 1002-3786(2005)04-0005-04 |
| 收稿时间： | 2005-08-10 |
| 修稿时间： | 2005-10-09 |
Preliminary Exploration to Induce Variations by Applying Colchicine to Adzuki Bean |
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| PU Shao-jing,LI Jin-yu,ZHANG Jing-jing,ZHAO Bo,JIN Wen-lin.Preliminary Exploration to Induce Variations by Applying Colchicine to Adzuki Bean[J].Journal of Beijing Agricultural College,2005,20(4):5-8. |
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| Authors: | PU Shao-jing LI Jin-yu ZHANG Jing-jing ZHAO Bo JIN Wen-lin |
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| Institution: | Beijing Agricultural College, Beijing 102206,China |
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| Abstract: | Sprouting seeds of Jingnong No.5 adzuki bean were treated with colchicine solution at 0.2000%,0.1000%,0.0500%,0.0250%,0.0125% and soaked for 8 h,16 h,24 h,respectively.The suitable time and concentration for inducing variations of adzuki bean with colchicine were probed into by the analysis of alive plant rate and plant height reduction rate in M_1 progeny,and by the analysis of range and variation coefficient and habitability of induced variations and obvious type of variation in M_(2)progeny.The results showed that:(1)On the basis of the alive plant rate,8 h 0.2000% and 24 h 0.1000% were the comparatively suitable treatment to induced variations;(2)Directeffect of concentration of treatment was obvious bigger than time of treatment;(3)As alive plant rate of 50% being the standard,the suitable concentrations were 0.2150%,0.156% and 0.115% in soaked times of 8 h,16 h and 24 h,respectively;(4)As plant height reducing for 50%~60% being the standard,the suitable concentrations were 0.0500%~0.2000% in the soaked times respectively;(5)There were two kinds of obvious variations of infected with powdery mildew and red-green piebald seed in M_(2)progeny,and its treatments were 8 h 0.0500% and 24 h 0.1000%,respectively. |
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| Keywords: | adzuki bean colchicine induced variation |
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