奶牛乳房炎主要致病菌在牛奶中的分布 |
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引用本文: | 曹立亭,胡松华.奶牛乳房炎主要致病菌在牛奶中的分布[J].安徽农业科学,2010,38(34):19541-19542. |
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作者姓名: | 曹立亭 胡松华 |
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作者单位: | [1]西南大学荣昌校区动物医学系,重庆荣昌402460 [2]浙江大学奶业科学研究所,浙江杭州310029 |
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基金项目: | 浙江省重大科技攻关农业项目,杭州市重大科技攻关项目 |
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摘 要: | 目的]观察奶牛乳房炎主要致病菌在牛奶中的分布情况。方法]在牛奶中添加一定量的乳房炎致病菌金黄色葡萄球菌或大肠杆菌,于4℃分别静置8、12和24 h,牛奶被分割成上、中、下3等份。采用平板菌落计数法测定各部分牛奶中的细菌数。结果]两种致病菌菌落数在牛奶中从下到上呈现上升趋势,且上部牛奶中的菌落数显著高于中、下部(P〈0.001),中部与下部差异不显著。结论]奶牛乳房炎致病菌经乳头管侵入后可以随牛奶中的脂肪滴上浮,进而侵入到乳腺的深层组织。
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关 键 词: | 奶牛乳房炎 金黄色葡萄球菌 大肠杆菌 分布 |
Distribution of Major Pathogenic Bacteria of Cow Mastitis in Milk |
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Institution: | CAO Li-ting et al(Department Veterinary,Rongchang Campus Southwest University,Rongchang,Chongqing 402460) |
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Abstract: | Objective]To observe the distribution of major pathogenic bacteria of cow mastitis in milk in vitro.Method]The Staphylococcus aureus and Escherichia coli were added into the milk.After standing for 8,12,24 h at 4 ℃,the milk were divided into upper,middle and lower three equal parts.The bacterial counts in the three parts of milk were respectively determined by plating colony-counting method.Result]The counts of these two pathogenic bacteria in upper milk were higher than that in middle and lower milk significantly(P<0.001),and no significant difference was found between middle milk and lower milk.Conclusion]The pathogenic bacteria of cow mastitis can be floating with fat droplet in milk and then invade into the deeper tissue of mammary gland. |
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Keywords: | Cow mastitis Staphylococcus aureus Escherichia coli Distribution |
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