| 我国引进萨罗罗非鱼群体的AFLP遗传指纹 |
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| 引用本文： | 赵金良,王伟伟,李思发,李学军,赵丽丽.我国引进萨罗罗非鱼群体的AFLP遗传指纹[J].上海水产大学学报,2007,16(3):293-296. |
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| 作者姓名： | 赵金良 王伟伟 李思发 李学军 赵丽丽 |
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| 作者单位： | [1]上海水产大学农业部水产种质资源与养殖生态重点开放实验室,上海200090 [2]中国水产科学研究院淡水渔业研究中心农业部水生动物遗传育种与养殖生物学重点开放实验室,江苏无锡214081 |
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| 基金项目： | 农业部水生动物遗传育种与养殖生物学重点开放实验室开放课题;上海市重点学科建设项目 |
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| 摘 要： | 应用AELP技术初步分析了我国引进的萨罗罗非鱼群体的遗传指纹特征，8对选择性引物在25个个体中共检测到247个扩增片段，每对引物扩增出的片段数目为27～34，平均扩增片段数目为30．9。群体的平均多态位点比例为16．6％。共检测出25个基因型，每个个体含有独自的基因型。群体内个体间的遗传相似度为0．929～0．995，群体遗传变异较小。本研究结果可为我国萨罗罗非鱼的种质保存与管理提供科学依据。
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| 关 键 词： | 萨罗罗非鱼 遗传指纹 遗传变异 |
| 文章编号： | 1004-7271（2007）03-0293-04 |
| 收稿时间： | 2006-09-04 |
| 修稿时间： | 2006-09-04 |
AFLP fingerprinting of the introduced stock of Sarotherodon melanotheron in China |
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| ZHAO Jin-liang,WANG Wei-wei,LI Si-fa,LI Xue-jun,ZHAO Li-li.AFLP fingerprinting of the introduced stock of Sarotherodon melanotheron in China[J].Journal of Shanghai Fisheries University,2007,16(3):293-296. |
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| Authors: | ZHAO Jin-liang WANG Wei-wei LI Si-fa LI Xue-jun ZHAO Li-li |
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| Abstract: | The genetic fingerprinting of the introduced stock of Sarotherodon melanotheron in China was analyzed using AFLP technique. A total of 247 fragments were detected in the 25 samples using eight primer pairs, the number of fragments produced by each pair of primers were 27 -34, the average fragments was 30.9, the average polymorphic loci of this stock were 16.6%. 25 genotypes were found with each sample owning one genotype. The genetic similarities of the introduced stock of S. melanotheron varied form 0.925 to 0. 995. These results could be served as genetic base for its management and conversation. |
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| Keywords: | AFLP |
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