| HPLC测定动物源性食品中倍他米松的残留 |
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| 引用本文： | 张晶,郭璐,赵兴然,张群,王向红.HPLC测定动物源性食品中倍他米松的残留[J].河北农业大学学报,2016(6):78-82. |
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| 作者姓名： | 张晶 郭璐 赵兴然 张群 王向红 |
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| 作者单位： | 河北农业大学 食品科技学院,河北 保定,071000 |
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| 基金项目： | 河北省科技支撑项目(152776120D) |
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| 摘 要： | 为建立检测动物性食品中倍他米松残留的高效液相色谱法。样品经乙酸乙酯提取后,进行液相色谱分析。采用Agilent C18(250mm×4.6mm,5μm)色谱柱,流动相为甲醇:水(60∶40,v∶v),流速为0.8mL/min,检测波长为240nm,柱温为30℃。方法的检出限和定量限分别为0.152μg/kg和0.502μg/kg。倍他米松在0.5~32μg/mL范围内线性关系良好,相关系数为0.998 9;选取5种动物源性食品进行倍他米松的加标回收试验,回收率在72.73%~93.06%之间,精密度RSD为3.78%(n=6)。本方法简便、可靠、重复性好,可用于动物性食品中倍他米松残留的检测。
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| 关 键 词： | 高效液相色谱 倍他米松 动物性食品 |
Determination of betamethasone in animal tissues by high performance liquid chromatography |
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| Abstract: | The research aims to establish a high performance liquid chromatography(HPLC)method to detect betamethasone in animal tissues.The sample was extracted by ethyl acetate and analyzed by HPLC.An Agilent C18(250 mm×4.6 mm,5μm)cartridge was used as chromatographic column. The mobile phase was methanol∶water (60∶40 ),flow rate 0.8 mL/min,detection wavelength 240 nm and column temperature 30℃.The limit of detection and quantity were 0.152 μg/kg and 0.502μg/kg.The average recovery rate of betamethasone in 5 animal tissues were in the range of 72.73%~93.06%with the precision of 3.78%(n=6).The method is simple,reliable and reproduc-ible,and it is suitable for the determination of betamethasone residue in food of animal tissues. |
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| Keywords: | HPLC betamethasone animal tissues |
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