| EMS诱导小麦TcLr19感叶锈病突变体的筛选 |
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| 引用本文： | 张维宏,安哲,范学锋,冯月琪,杨文香,刘大群.EMS诱导小麦TcLr19感叶锈病突变体的筛选[J].华北农学报,2016(4):88-93. |
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| 作者姓名： | 张维宏 安哲 范学锋 冯月琪 杨文香 刘大群 |
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| 作者单位： | 河北农业大学 植物保护学院，河北省农作物病虫害生物防治工程技术研究中心，河北 保定 071001 |
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| 基金项目： | 国家重点基础研究发展计划项目(2013CB127700)；河北省现代农业产业体系小麦产业创新团队建设项目 |
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| 摘 要： | 为了利用小麦感叶锈病突变体进行抗病基因功能和机制研究,用EMS处理小麦抗叶锈病近等基因系Tc Lr19的种子,对获得的M2、M3植株进行农艺性状观察和田间抗叶锈性鉴定。结果显示,不同浓度的EMS溶液处理种子共获得367个M1单株,处理浓度为1.0%时,接近半致死剂量,且M2表型突变频率最高,适宜突变筛选。在2 359个M2突变群体中,共筛选到表型突变体38个,表型突变频率1.61%;筛选出感叶锈病突变体53个,感病突变频率2.25%。在459个M3感病突变群体中,共鉴定出146个感病突变体,其中M36-2、M333-8、M333-9、M333-11、M344-4、M396-8这6个M3感病突变材料,遗传稳定率较高,达70%以上。为保证结果的可靠性,试验中还利用Lr19的分子标记对突变体进行分子辅助筛选。结果表明,EMS诱变是获得小麦感叶锈病突变体的有效手段,不仅为小麦抗叶锈基因的克隆和功能研究提供了重要的基础材料,还为小麦育种提供了新的种质。
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| 关 键 词： | 小麦 叶锈病 甲基磺酸乙酯 突变体 |
Screening of Mutants Susceptible to Puccina triticina in TcLr19 Induced by EMS |
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| Abstract: | In order to study the function and mechanism of the resistance gene of wheat leaf rust,seeds of wheat TcLr19 was treated by EMS.The leaf rust resistance and agronomic traits were investigated and phenotypic mutants were identified in M1 and M2 .A total of 367 individual plants were obtained in M1 with different concentrations of EMS.According to the emergence rate,seed rate and mutation rate,EMS 1 .0% was considered the optimum con-centration.In 2 359 M2 mutant,a total of 38 phenotype mutations were screened out with 1 .61 % mutation rate,and a total of 53 susceptible mutations to Puccinia triticina were screened out with 2.25% mutation rate.A total of 146 susceptible plants were obtained in 459 M3 susceptible populations,especially M3 6-2,M3 33-8,M3 33-9,M3 33-1 1 ,M3 44-4 and M3 96-8,and the frequency of susceptible mutation were more than 70%.Furthermore,Lr19 molecular marker-assisted selection was also used in order to guarantee the reliability of the results in the test.The results showed that EMS treatment was an effective method in screening of mutants susceptible to P.triticina.The result not only provided important genetic resources for wheat leaf rust resistance gene cloning and functional genomic re-search,but also provided new germplasms in wheat breeding. |
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| Keywords: | Wheat Leaf rust Ethyl methane sulfonate (EMS) Mutant |
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