| 蓝刺头多糖体外抗氧化及抗菌活性的研究 |
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| 引用本文： | 杨斌,萨茹丽,张月梅,达来宝力格,宋越,宋爱军,陈伟,卢岩,刘敏,杨英,赵世华.蓝刺头多糖体外抗氧化及抗菌活性的研究[J].华北农学报,2016(4):233-238. |
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| 作者姓名： | 杨斌 萨茹丽 张月梅 达来宝力格 宋越 宋爱军 陈伟 卢岩 刘敏 杨英 赵世华 |
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| 作者单位： | 1. 内蒙古农牧业科学院 兽医研究所,内蒙古 呼和浩特,010031;2. 内蒙古农业大学 动物科学学院,内蒙古 呼和浩特,010018;3. 内蒙古农业大学 兽医学院,内蒙古 呼和浩特,010018 |
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| 基金项目： | 内蒙古自治区自然科学基金项目(2016BS(LH)0301)；内蒙古农牧业创新基金项目(2013CXJJM05) |
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| 摘 要： | 通过研究蓝刺头粗多糖及其洗脱纯化组分蓝刺头多糖A、B、C的体外抗氧化和抗菌作用,初步确定蓝刺头多糖的生物活性。结果显示,添加浓度相同时,蓝刺头粗多糖的总还原能力以及清除DPPH有机自由基能力最强,显著高于蓝刺头多糖A,极显著高于蓝刺头多糖B、C。当添加浓度为0.1~0.3 mg/m L时,蓝刺头粗多糖清除羟基自由基的能力略高于蓝刺头多糖A,但差异不显著;当添加浓度为0.4 mg/m L时,蓝刺头粗多糖清除羟基自由基的能力略高于蓝刺头多糖A,蓝刺头多糖A略高于蓝刺头多糖B,三者差异不显著,但均极显著高于蓝刺头多糖C,当添加浓度为0.5 mg/m L时,蓝刺头粗多糖清除羟基自由基的能力显著高于蓝刺头多糖A、B,极显著高于蓝刺头多糖C。试验条件下,蓝刺头粗多糖和蓝刺头多糖A对大肠杆菌为低度敏感,最低抑菌浓度均为0.62 mg/m L,蓝刺头多糖B和C对大肠杆菌不敏感,最小抑菌浓度均为1.25 mg/m L;蓝刺头粗多糖、蓝刺头多糖A、B和C对金黄色葡萄球菌为不敏感,最低抑菌浓度分别为1.25,2.50,2.50,2.50 mg/m L;蓝刺头粗多糖、蓝刺头多糖B、C对铜绿假单胞菌为不敏感,最低抑菌浓度均为5.00 mg/m L,蓝刺头多糖A对铜绿假单胞菌无抑制作用;蓝刺头粗多糖和蓝刺头多糖A、B、C对沙门氏菌均为不敏感,最低抑菌浓度分别为2.50,2.50,1.25,5.00 mg/m L。说明蓝刺头粗多糖的抗氧化能力最强,其次为蓝刺头多糖A,而蓝刺头多糖B和C的抗氧化能力较弱。蓝刺头粗多糖和蓝刺头多糖A具有一定的抗大肠杆菌活性,蓝刺头粗多糖和蓝刺头多糖A、B、C均无抗金黄色葡萄球菌、铜绿假单胞菌以及沙门氏菌活性。
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| 关 键 词： | 蓝刺头 多糖 抗氧化活性 抗菌活性 |
Study on Antioxidant and Antibacterial Activity in vitro of Echinops latifolius Tausch Polysaccharides |
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| Abstract: | In this study antioxidant activity and antibacterial activity of Echinops latifolius Tausch polysaccha-rides was researched.The result showed that when the initial concentrations were same,ETCP has a highest effect of total reducing capacity,clearance of DPPH free radical in vitro,higher than ETPA,significantly higher than ETPB and ETPC.When the added concentrations were 0.1 -0.3 mg /mL,ETCP has a little higher scavenging effects on free radicals than that of ETPA,but no obvious difference;when the added concentrations were 0.4 mg /mL,ETCP has a little higher scavenging effects on free radicals than that of ETPA,ETPA has a little higher scavenging effects than that of ETPB,and difference were not significant,they were significantly higher than that ETPC,when the add-ed concentrations were 0.5 mg /mL,ETCP has a higher scavenging effects on free radicals than that of ETPA,ET-PB,significantly higher than ETPC.Experimental condition,ETCP and ETPA were less sensitive to E.coli,the mini-mal inhibitory concentration(MIC)were 0.62 mg /mL,ETPB and ETPC were insensitive the MIC were 1.25 mg /mL;ETCP,ETPA,ETPB and ETPC were insensitive to Staphylococcus aureus,the MIC were 1.25,2.50,2.50,2.50 mg /mL respectively;ETCP,ETPB and ETPC were insensitive to Pseudomonas aeruginosa,the MIC were 5.00 mg /mL,ETPA had no inhibition to Pseudomonas aeruginosa;ETCP,ETPA,ETPB and ETPC were insensitive to Salmonella,the MIC were 2.50,2.50,1 .25,5.00 mg /mL respectively.It means ETCP has the strongest antioxidant effect and sec-ondly for ETPA,ETPB and ETPC were weak.ETCP and ETPA has a certain resistance to activity of E.coli,and ET-PA,ETPB and ETPC has no antibacterial action to Staphylococcus aureus,Pseudomonas aeruginosa and Salmonella. |
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| Keywords: | Echinops latifolius Tausch Polysaccharide Antioxidant activity Antibacterial activity |
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