| ~(252)Cf裂变中子辐照玉米种子生物学效应初步研究 |
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| 引用本文： | 刘忠祥,徐大鹏,姚泽恩,寇思荣,王晓娟,杨彦忠,连晓荣,周玉乾,何海军,李志明.~(252)Cf裂变中子辐照玉米种子生物学效应初步研究[J].华北农学报,2016(5):71-77. |
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| 作者姓名： | 刘忠祥 徐大鹏 姚泽恩 寇思荣 王晓娟 杨彦忠 连晓荣 周玉乾 何海军 李志明 |
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| 作者单位： | 1. 甘肃省农业科学院 作物研究所,甘肃 兰州,730070;2. 兰州大学 核科学与技术学院，甘肃 兰州 730000; 兰州大学教育部中子应用技术工程研究中心，甘肃 兰州 730000 |
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| 基金项目： | 甘肃省科技重大专项(0801 NKDA016)，甘肃省农科院农业科技创新专项(2011 GAAS07)，兰州大学中央高校基本科研业务费专项资金项目(lzujbky-2015-bt07) |
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| 摘 要： | 为了探索快中子辐照玉米种子的生物学效应,筛选适宜的中子辐照剂量,以获得基因定位、克隆及其功能分析的突变体材料,采用252Cf裂变中子源对玉米自交系LY8405和PH6WC种子进行辐照,结果表明,低吸收剂量(0.36~4.19 Gy)的中子辐照玉米干种子后,对自交系LY8405的出苗率有一定的抑制作用,对自交系PH6WC的出苗率有一定的促进作用;2份材料M1畸变率、变异率、遗传总变异率,随着辐照剂量的增加有逐步升高的趋势,其中辐照剂量为4.19 Gy时对LY8405最为有效,畸变率、变异率、遗传总变异率分别为27.8%,38.9%,66.7%;辐照剂量为2.48 Gy时对PH6WC最为有效,畸变率、变异率、遗传总变异率分别为7.1%,14.3%,21.4%;M2株高、穗位、叶片数、雄穗分枝数、穗长、穗粗、穗行数、轴粗等主要农艺性状变异随辐照剂量的增加,其变异系数也随之增加。研究表明,不同基因型玉米种子对快中子辐射剂量的敏感性不同,初步认为2.00~4.19 Gy的辐照剂量是玉米252Cf裂变中子辐照处理的适宜剂量。
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| 关 键 词： | 玉米 252 Cf 中子 生物学效应 辐射剂量 |
Preliminary Study on Biological Effects of Maize Seeds Irradiated Using 252 Cf Fission Neutron Source |
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| Abstract: | To study the biological effects and rules of neutron irradiation on maize seeds.Maize inbred lines LY8405 and PH6WC were irradiated by using252 Cf fission neutron source,screening suitable neutron irradiation dose and creating mutants for gene mapping,cloning and its functional analysis.The results showed that the germination rate of LY8405 was descended after neutron irradiation at low absorbed doses(0.36 -4.1 9 Gy),however that of PH6WC was increased.With irradiation dose increasing,the rates of distortion,mutation and total genetic variation of the two materials M1 were all added.LY8405 showed the most sensitively biological effects at 4.1 9 Gy irradiation dose,the rates of distortion,mutation and total genetic variation were 27.8%,38.9% and 66.7%,respectively;PH6WC showed the most sensitive effect at 2.48 Gy irradiation dose,the rates of distortion,mutation and total ge-netic variation were 7.1 %,1 4.3% and 21 .4%,respectively.Besides,the coefficient of variation of the agronomic characters,including plant height,ear position,leaf number,tassel branch number,spike length,ear diameter,kernel rows per spike and axis width,were all raised with the rising of irradiation dose.The paper indicated that the sensi-
tive degree of maize genotype was different to fast neutron radiation dose;initially considered that 2.00 -4.1 9 Gy was suitable dose of irradiate maize seed using252 Cf fission neutron source. |
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| Keywords: | Maize 252 Cf Neutron Biology effects Irradiating doses |
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