Rhizosphere pH Profile of Rice Plant Influenced by Al Treatment |
| |
| Authors: | Yoshitake Hayashi Keitaro Tanoi Hiroyuki Nishiyama Tomoko M Nakanishi |
| |
| Institution: | Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo, 113–8657 Japan |
| |
| Abstract: | To study the mechanisms of Al tolerance in rice, we focused on the change of rhizosphere pH. The 4-d seedlings were treated with Al solution (0, 10, 50 mM) for 24 h. Then each sample was put on an agarose gel including bromocresol green, so that the color of the gel indicated pH change. During 2-h contact, the pH of rhizosphere was decreased gradually, especially for Al-treated samples, showing the specific pH profiles along the root axis. Pretreatment of sample plants with a decoupling reagent 2,4-dinitrophenol (DNP) or a plasma membrane H+-ATPase inhibitor Na3VO4 did not decrease rhizosphere pH. Therefore, it was suggested that the H+ secretion activity was involved with Al-tolerant mechanisms of rice. |
| |
| Keywords: | aluminum H+-ATPase rhizosphere pH rice root |
|
|
