| 两株益生菌的部分生物学特性研究 |
| |
| 引用本文： | 朱宏亮,陈德龙,许光勇,姚紫彤,任晓明.两株益生菌的部分生物学特性研究[J].北京农学院学报,2011,26(1):23-26. |
| |
| 作者姓名： | 朱宏亮 陈德龙 许光勇 姚紫彤 任晓明 |
| |
| 作者单位： | 北京农学院动物科技学院,北京,102206 |
| |
| 基金项目： | 北京市自然科学基金，北京市教委资助项目 |
| |
| 摘 要： | 为了探明2株植物源芽孢杆菌K,F（暂定名）的抗逆性与抑菌特性,对其进行了耐酸,耐胆盐与抑菌试验。用pH 1.5、2.0、3.5的人工胃液模拟胃液环境;用0.3%胆盐及0.1%CuSO4.5H2O的人工肠液模拟肠液环境。分别测定K,F的培养上清与菌液抑制大肠杆菌的能力。采用传统的表型鉴定及生理生化鉴定方法及16SrDNA方法对K,F进行鉴定。结果：经过pH 1.5、2.0、3.5的胃液处理,K,F均有较高的存活率,随着pH的上升,存活率有上升的趋势;耐胆盐试验,处理组细菌存活率有所下降,但均高于90%;K的培养上清液和菌液对大肠杆菌的抑菌环直径为14.33,18.57 mm,F的为14.11,15.68 mm。所以人工胃液,人工肠液对K,F的活菌数几乎不产生影响。K,F的培养上清和菌液对大肠杆菌有很好的抑制作用。综合鉴定结果K为枯草芽孢杆菌,F为地衣芽孢杆菌。
|
| 关 键 词： | 芽孢杆菌 抗逆性 抑菌 16SrDNA 鉴定 |
The study of partial biological characteristics and identification on two plant probiotics for animals |
| |
| ZHU Hong-liang,CHEN De-long,XU Guang-yong,YAO Zi-tong,REN Xiao-ming.The study of partial biological characteristics and identification on two plant probiotics for animals[J].Journal of Beijing Agricultural College,2011,26(1):23-26. |
| |
| Authors: | ZHU Hong-liang CHEN De-long XU Guang-yong YAO Zi-tong REN Xiao-ming |
| |
| Institution: | ZHU Hong-liang,CHEN De-long,XU Guang-yong,YAO Zi-tong,REN Xiao-ming(College of Animal Science and Technology,Beijing Universty of Agriculture,Beijing 102206,China) |
| |
| Abstract: | In order to study the stress resistance of two plant Bacillus K and F(temporary names),acid resistant and bile tolerance experiment had been made.Simulated stomach juice was pH 1.5,2.0 and 3.5,simulated intestinal fluid included 0.3% bile salt and 0.1% CuSO4 5H2O.The K and F's antibacterial capacity of supernatant and bacterial was liquid tested respectively.They were identified with phenotype,biochemical and 16SrDNA methods.In artifical stomach of pH 1.5,2.0,and 3.5,both K and F had high survival rates.Wit... |
| |
| Keywords: | 16SrDNA |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
