| 果蔬复合固体饮料的制备及其对小鼠肝性骨病的预防作用 |
| |
| 引用本文： | 夏禹承,吴淑娟,张润光,张有林.果蔬复合固体饮料的制备及其对小鼠肝性骨病的预防作用[J].农业工程学报,2023,39(3):248-259. |
| |
| 作者姓名： | 夏禹承 吴淑娟 张润光 张有林 |
| |
| 作者单位： | 陕西师范大学食品工程与营养科学学院,西安 710119 |
| |
| 基金项目： | 中央财政林业科技推广示范项目(SLTG[2021]04）;陕西省重点研发计划项目（2022NY-018）;陕西省林业科学科技创新专项（SXLK2020-02-04） |
| |
| 摘 要： | 为开发一种感官营养俱佳的保健食品,该研究以欧李、猕猴桃、胡萝卜、葡萄、柑橘5种果蔬为原料,用喷雾干燥法制备复合固体饮料（以下简称“五果粉”）,后以五果粉饲喂雌性C57小鼠,测定血清指标,分析骨微结构,探究其对高脂饮食诱导的小鼠肝性骨病的预防作用,为五果粉的制作和营养保健功能提供依据。结果表明：5种果蔬的配方比例为40%欧李、20%猕猴桃、20%胡萝卜、10%柑橘、10%葡萄。添加可溶性固形物总量的40%的复合助干剂（麦芽糊精∶分离乳清蛋白=30∶10）与3%的稳定剂羧甲基纤维素钠,喷雾干燥条件：进风温度126℃、进料速度6.3m L/min、转子流量计高度44.00mm、可溶性固形物含量11.00%,在该条件下生产的五果粉集粉率60.86%。血清试验结果表明,给药组小鼠血清中骨钙素和抗酒石酸酸性磷酸酶5b的水平显著低于模型组（P<0.05）;超氧化物歧化酶和谷胱甘肽过氧化物酶水平显著高于模型组（P<0.05）,丙二醛水平显著低于模型组（P<0.05）;谷丙转氨酶、谷草转氨酶水平显著低于模型组（P<0.05）,且与空白组相比无显著差异（P>0.05）;总胆固...
|
| 关 键 词： | 饮料 干燥 果蔬 复合固体饮料 小鼠高脂饮食 肝性骨病预防 |
| 收稿时间： | 2022/9/22 0:00:00 |
| 修稿时间： | 2022/11/14 0:00:00 |
Preparation of fruit-vegetable composite solid beverages and its preventive effect on high-fat diet-induced hepatic bone disease in mice |
| |
| XIA Yucheng,WU Shujuan,ZHANG Runguang,ZHANG Youlin.Preparation of fruit-vegetable composite solid beverages and its preventive effect on high-fat diet-induced hepatic bone disease in mice[J].Transactions of the Chinese Society of Agricultural Engineering,2023,39(3):248-259. |
| |
| Authors: | XIA Yucheng WU Shujuan ZHANG Runguang ZHANG Youlin |
| |
| Institution: | College of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi''an 710119, China |
| |
| Abstract: | Hepatic osteodystrophy is one of the metabolic skeletal diseases, characterized by global changes in bone mineral density in patients with chronic liver disease (CLD) under osteomalacia and osteoporosis. It is often overlooked the complication in patients with CLD. Hepatic osteodystrophy can seriously affect the quality of life and long-term prognosis of the patients. In this study, five kinds of fruits and vegetables (including cerasus humilis, kiwifruit, carrot, grape, and orange) were used as the raw materials to prepare a composite solid beverage (hereinafter referred to as "Wuguo Powder") by spray drying. Then, the female C57 mice were fed with the Wuguo powder, in order to determine the serum indexes and the bone microstructures. A systematic investigation was made to clarify the preventive effects of Wuguo Powder on the hepatic osteopathy induced by high-fat diet. The basis was also offered for the production and the nutrition health function of the Wuguo Powder. The results showed that the optimal formula for five kinds of fruits and vegetables was 40% cerasus humilis, 20% kiwifruit, 20% carrot, 10% orange, and 10% grape. The ingredients were added 40% of the total soluble solids composite drying agent (maltodextrin: WPI=30:10) and 3% of the stabilizer sodium carboxymethyl cellulose (CMC). The spray drying conditions were obtained: the air temperature of entry was 126°C, the feed rate was 6.3 mL/min, the height of the rotameter was 44.00mm, and the soluble solid content was 11.00%. The powder collection rate of Wuguo Powder was 60.86% under these conditions. The serum test showed that the levels of OC and TRACP-5b in the serum of the mice in the administration group were significantly lower than those in the model group (P<0.05). The levels of SOD and GSH-Px were significantly higher than those in the model group (P<0.05), while the levels of MDA were significantly higher than those in the model group (P<0.05), whereas, the lower than that in the model group (P<0.05). The ALT and AST levels were significantly lower than that in the model group (P<0.05), indicating no significant difference, compared with the blank group (P>0.05). The TC, TG, LDL-C levels were significantly lower than that in the model group (P<0.05). The level of HDL-C was significantly higher than that in the model group (P<0.05), indicating no significant difference from the blank group (P>0.05). The Micro-CT showed that the femoral BMD, BV/TV, BS/TV, Tb.N, and Conn.D of the administration group significantly increased (P<0.05), compared with the model group, whereas, the BS/BV and Tb.Sp significantly decreased (P<0.05). The results showed that five fruit powders were prepared by spray drying using the cerasus humilis, kiwi fruit, carrot, orange, and grape as the raw materials, adding the maltodextrin, whey protein isolate (WPI) as a composite drying agent and stabilizer CMC. Five kinds of fruit-vegetable complex solid drinks regulated bone metabolism. Specifically, the levels of OC and TRACP-5b decreased in the serum, and the oxidative stress was improved by the decreasing content of MDA and the increasing levels of SOD and GSH-Px in the serum. The liver damage was relieved by the decreasing levels of ALT and AST in the serum, and the blood lipid metabolism was regulated by the decreasing levels of TG, TC, LDL-C in the serum and the increasing level of HDL-C in the serum. As such, the bone microstructure was improved by increasing BMD, BV/TV, BS/TV, Tb.N, and Conn.D value, while decreasing BS/BV, and Tb.Sp. Therefore, five kinds of fruit-vegetable complex solid drinks can be expected to serve as the better preventive effect on the hepatic osteopathy induced by a high-fat diet in mice. |
| |
| Keywords: | beverages drying fruits and vegetables composite solid beverages high-fat diet for mice prevention of hepatic bone disease |
|
| 点击此处可从《农业工程学报》浏览原始摘要信息 |
| 点击此处可从《农业工程学报》下载免费的PDF全文 |
|
